Identification on Osteopontin Promoter Gene Polymorphism and Post-thawing Quality in Dairy Bull Peranakan Friesian Holstein

9th Annual Basic Science International Conference 2019

自然科学（总论）

Hernawati, Tatik^1 ; Mulyati, Sri^1 ; Rimayanti^1 ; Suprayogi, Tri Wahyu^1

Faculty of Veterinary Medicine, Airlangga University, Mulyorejo Surabaya, Indonesia^1

关键词: Cryo-preservation;    diary bull Friesian Holstein;    Gene polymorphism;    Mutation sites;    Nucleotide basis;    Osteopontin;    Semen qualities;    spermatozoa;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/546/6/062010/pdf
DOI  :  10.1088/1757-899X/546/6/062010

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

This study aims to determine the existence of osteopontin promoter gene polymorphism in dairy bull Peranakan Holstein Friesian (PFH) and its relationship with the quality of PFH bull frozen semen. A total of 10 Holstein Friesian dairy cow blood samples were taken and then DNA extracted and amplified using SPP1F and SPP1R primers. The target band 306bp was detected in all samples and continued by sequencing to analyze the nucleotide bases. The results showed that sample with low quality frozen semen were deleted in the 10098 base and a transition (T-C) in bases 10054. The results indicated that this mutation site could be related to the trait susceptibility to frozen semen quality. Comprehensive studies are highly needed to address other parameters related to any abnormality in sperm during cryopreservation.