Relationship between the Apolipoprotein AI, B gene polymorphism and the risk of non-traumatic osteonecrosis

Lipids in Health and Disease

Zhong-Tang Liu²  Yan-Jie Guo²  Shi-Chang Zhao²  Zhao Liu¹  Ji-Min Yin² 

[1] Department of hematology, Shanghai Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China;Department of Orthopedic Surgery, Shanghai Sixth People’s Hospital, Shanghai Jiaotong University, No.600 Yishan Road, Shanghai 200233, China

关键词: Molecular epidemiology;    Gene polymorphism;    Non-traumatic osteonecrosis;    Apolipoprotein B;    Apolipoprotein AI;   

Others  :  1152047
DOI  :  10.1186/1476-511X-13-149

 received in 2014-05-18, accepted in 2014-08-30,  发布年份 2014

【 摘 要 】

Background

Previous studies suggested that Apolipoprotein AI (ApoAI) and apolipoprotein B (ApoB) gene polymorphisms may result in lipid metabolism disorders. Genetic polymorphisms in these genes may be associated with the occurrence of osteonecrosis.

Methods

We designed a case-control study including 429 patients of osteonecrosis and 368 age- and sex-matched control subjects. Polymerase chain reaction was used to amplify the DNA fragments in promoter -75 G > A of ApoAI gene and EcoR I, Xba I and 3′-VNTR of ApoB gene in osteonecrosis patients and healthy controls. We utilized polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to genotype these four single nucleotide polymorphisms (SNPs).

Results

For -75 G > A polymorphism of ApoAI, AA genotype frequency (0.501) was significantly higher in patients with osteonecrosis than that in control (0.462) subjects (P <0.001), GA genotype frequency (0.170) was significantly lower than that in the control (0.310) group (P <0.0001). In osteonecrosis patients, the odds ratio (OR) of A allele was 3.932 (95% CI: 3.0847 ~ 5.0123), which suggested that subjects carrying A allele of promoter region -75 G > A of ApoAI gene had higher susceptibility to osteonecrosis than G allele carriers. The genotype and allele frequency distributions showed no significant difference in EcoR I, Xba Iand 3′-VNTR loci of ApoB gene between the osteonecrosis group and control group.

Conclusion

Our study suggested that ApoAI gene -75G > A polymorphism may be associated with susceptibility to osteonecrosis in Chinese population. However, our results need further investigation with large sample size and various populations.

【 授权许可】

   
2014 Yin et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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