Asymptomatic Plasmodium vivax infections induce robust IgG responses to multiple blood-stage proteins in a low-transmission region of western Thailand

Malaria Journal

Research

Christopher L. King¹  Wang Nguitragool²  Patiwat Sa-angchai³  Rick M. Fairhurst⁴  Chalermpon Kumpitak⁵  Jetsumon Sattabongkot⁵  Julian C. Rayner⁶  Jessica B. Hostetler⁷  Anjali Yadava⁸  Jakub Gruszczyk⁹  Camila T. França^1,10  Wai-Hong Tham^1,10  Ivo Mueller^1,11  Michael T. White^1,12  Rhea J. Longley^1,13 

[1] Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH, USA;Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA;Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;Malaria Programme, Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK;Malaria Programme, Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK;Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA;Malaria Vaccine Branch, United States Military Malaria Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA;Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia;Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia;Department of Medical Biology, University of Melbourne, Melbourne, Australia;Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia;Department of Medical Biology, University of Melbourne, Melbourne, Australia;ISGlobal, Barcelona Institute for Global Health, Hospital Clínic-Universitat de Barcelona, 08036, Barcelona, Spain;Institut Pasteur, Paris, France;Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia;Imperial College, London, UK;Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia;Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;Department of Medical Biology, University of Melbourne, Melbourne, Australia;

关键词: Plasmodium vivax;    Malaria;    IgG;    Antibody;    Humoral immunity;    Vaccine;    Elimination;    Asymptomatic;    Exposure;   

DOI  :  10.1186/s12936-017-1826-8

 received in 2017-01-21, accepted in 2017-04-19,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThailand is aiming to eliminate malaria by the year 2024. Plasmodium vivax has now become the dominant species causing malaria within the country, and a high proportion of infections are asymptomatic. A better understanding of antibody dynamics to P. vivax antigens in a low-transmission setting, where acquired immune responses are poorly characterized, will be pivotal for developing new strategies for elimination, such as improved surveillance methods and vaccines. The objective of this study was to characterize total IgG antibody levels to 11 key P. vivax proteins in a village of western Thailand.MethodsPlasma samples from 546 volunteers enrolled in a cross-sectional survey conducted in 2012 in Kanchanaburi Province were utilized. Total IgG levels to 11 different proteins known or predicted to be involved in reticulocyte binding or invasion (ARP, GAMA, P41, P12, PVX_081550, and five members of the PvRBP family), as well as the leading pre-erythrocytic vaccine candidate (CSP) were measured using a multiplexed bead-based assay. Associations between IgG levels and infection status, age, and spatial location were explored.ResultsIndividuals from a low-transmission region of western Thailand reacted to all 11 P. vivax recombinant proteins. Significantly greater IgG levels were observed in the presence of a current P. vivax infection, despite all infected individuals being asymptomatic. IgG levels were also higher in adults (18 years and older) than in children. For most of the proteins, higher IgG levels were observed in individuals living closer to the Myanmar border and further away from local health services.ConclusionsRobust IgG responses were observed to most proteins and IgG levels correlated with surrogates of exposure, suggesting these antigens may serve as potential biomarkers of exposure, immunity, or both.

【 授权许可】

CC BY   
© The Author(s) 2017