Subclass responses and their half-lives for antibodies against EBA175 and PfRh2 in naturally acquired immunity against Plasmodium falciparum malaria

Malaria Journal

Research

Roseangela Nwuba¹  Michael T White²  Mats Wahlgren³  Hodan Ahmed Ismail³  Muyideen K Tijani⁴  Kristina EM Persson⁵  Christine Langer⁶  Linda Reiling⁶  James G Beeson⁶ 

[1] Cellular Parasitology Programme, Cell Biology and Genetics Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria;Department of Infectious Disease Epidemiology, Imperial College, London, UK;Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Nobels väg 16, 17177, Stockholm, Sweden;Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Nobels väg 16, 17177, Stockholm, Sweden;Cellular Parasitology Programme, Cell Biology and Genetics Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria;Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Nobels väg 16, 17177, Stockholm, Sweden;Department of Laboratory Medicine, Lund University, University Hospital, Lund, Sweden;The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, VIC, Australia;

关键词: Plasmodium falciparum;    Malaria;    EBA175;    PfRh2;    Merozoite;    IgG;    Subclass;    Antibody;    HbAA;    HbAS;   

DOI  :  10.1186/1475-2875-13-425

 received in 2014-07-09, accepted in 2014-10-25,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPlasmodium falciparum EBA175 and PfRh2 belong to two main families involved in parasite invasion, and both are potential vaccine candidates. Current knowledge is limited regarding which target antigens and subclasses of antibodies are actually important for protection, and how naturally acquired immunity is achieved.MethodsRepeated blood samples were collected from individuals in Nigeria over a period of almost one year. ELISA was used to analyse subclasses of IgG responses.ResultsFor both EBA175 (region III-V) and (a fragment of) PfRh2, the dominant antibody responses consisted of IgG1 and IgG3 followed by IgG2, while for PfRh2 there was also a relatively prominent response for IgG4. High levels of IgG1, IgG2 and IgG3 for EBA175 and total IgG for PfRh2 correlated significantly with a lower parasitaemia during the study period. Children with HbAS had higher levels of some subclasses compared to children with HbAA, while in adults the pattern was the opposite. The half-lives of IgG2 and IgG4 against EBA175 were clearly shorter than those for IgG1 and IgG3.ConclusionEBA175 and PfRh2 are potential targets for protective antibodies since both correlated with lower parasitaemia. The shorter half-lives for IgG2 and IgG4 might explain why these subclasses are often considered less important in protection against malaria. Triggering the right subclass responses could be of critical importance in a successful vaccine. Further studies are needed to evaluate the role of haemoglobin polymorphisms and their malaria protective effects in this process.

【 授权许可】

Unknown   
© Ahmed Ismail et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.