A rabbit model for experimental alveolar cleft grafting

Journal of Translational Medicine

Research

Peter Kessler¹  Mohammad Kamal²  Felix Gremse³  Bernd Lethaus⁴  Frank Hölzle⁴  Alexander Bartella⁴  Lars Andersson⁵  Rene Tolba⁶ 

[1]Department of Cranio-Maxillofacial Surgery, Maastricht University Medical Center, Postbus 5800, P. Debyelaan, 6202 AZ, Maastricht, The Netherlands

[2]Department of Cranio-Maxillofacial Surgery, Maastricht University Medical Center, Postbus 5800, P. Debyelaan, 6202 AZ, Maastricht, The Netherlands

[3]Department of Oral and Maxillofacial Surgery, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

[4]Department of Experimental Molecular Imaging, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

[5]Department of Oral and Maxillofacial Surgery, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

[6]Department of Surgical Sciences, Faculty of Dentistry, Health Sciences Center, Kuwait University, 13110, Safat, Kuwait

[7]Institute for Laboratory Animal Science and Experimental Surgery, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

关键词: Animal testing;    Cleft lip and palate;    Grafting;    Tissue-engineering;    Rabbit;   

DOI  :  10.1186/s12967-017-1155-2

 received in 2017-01-18, accepted in 2017-02-21,  发布年份 2017

来源: Springer

【 摘 要 】

ObjectivesThe purpose of the present study was to develop an animal model for creating alveolar cleft defects with properly simulated clinical defect environment for tissue-engineered bone-substitute materials testing without compromising the health of the animal. Cleft creation surgery was aimed at creating a complete alveolar cleft with a wide bone defect with an epithelial lining (oral mucosa) overlying the cleft defect.MethodsA postmortem skull of a New Zealand White (NZW) rabbit skull (Oryctolagus cuniculus) underwent an osteological and imaging survey. A pilot postmortem surgery was conducted to confirm the feasability of a surgical procedure and the defect was also radiologically confirmed and illustrated with micro-computed tomography. Then, a surgical in vivo model was tested and evaluated in 16 (n = 16) 8-week-old NZW rabbits to create in vivo alveolar cleft creation surgery.ResultsClinical examination and imaging analysis 8 weeks after cleft creation surgery revealed the establishment of a wide skeletal defect extending to the nasal mucosa simulating alveolar clefts in all of the rabbits.ConclusionsOur surgical technique was successful in creating a sizable and predictable model for bone grafting material testing. The model allows for simulating the cleft site environment and can be used to evaluate various bone grafting materials in regard to efficacy of osteogenesis and healing potential without compromising the health of the animal.

【 授权许可】

CC BY   
© The Author(s) 2017