In Vivo Application of Tissue-Engineered Veins Using Autologous Peripheral Whole Blood: A Proof of Concept Study

EBioMedicine

卷:1

Anders Jeppsson¹  Robert Saalman²  Helena Borg²  Suchitra Sumitran-Holgersson³  Michael Olausson³  Vijay Kumar Kuna³  Pradeep B. Patil³  Galyna Travnikova³  Henrik Bäckdahl⁴ 

[1] Department of Cardiothoracic Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden;

[2] Department of Paediatrics, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden;

[3] Department of Surgery, University of Gothenburg, Sahlgrenska University Hospital, Gothenburg, Sweden;

[4] SP Technical Research Institute of Sweden, Dept. of Chemistry, Materials and Surfaces, SE-50462 Borås, Sweden;

关键词: Vein conduits;    Vascular diseases;    Tissue-engineering;    Endothelial precursors;   

DOI  :  10.1016/j.ebiom.2014.09.001

来源: DOAJ

【 摘 要 】

Vascular diseases are increasing health problems affecting >25 million individuals in westernized societies. Such patients could benefit from transplantation of tissue-engineered vascular grafts using autologous cells. One challenge that has limited this development is the need for cell isolation, and risks associated with ex vivo expanded stem cells. Here we demonstrate a novel approach to generate transplantable vascular grafts using decellularized allogeneic vascular scaffolds, repopulated with peripheral whole blood (PWB) in vitro in a bioreactor. Circulating, VEGFR-2+/CD45+ and a smaller fraction of VEGFR-2+/CD14+ cells contributed to repopulation of the graft. SEM micrographs showed flat cells on the luminal surface of the grafts consistent with endothelial cells. For clinical validation, two autologous PWB tissue-engineered vein conduits were prepared and successfully used for by-pass procedures in two pediatric patients. These results provide a proof of principle for the generation of transplantable vascular grafts using a simple autologous blood sample, making it clinically feasible globally.

【 授权许可】

Unknown