Bone regeneration using composite non-demineralized xenogenic dentin with beta-tricalcium phosphate in experimental alveolar cleft repair in a rabbit model

Journal of Translational Medicine

Research

Peter Kessler¹  Mohammad Kamal²  Felix Gremse³  Stefanie Rosenhain³  Bernd Lethaus⁴  Frank Hölzle⁴  Alexander K. Bartella⁴  Adel Al-Asfour⁵  Lars Andersson⁵  Rene Tolba⁶ 

[1]Department of Cranio-Maxillofacial Surgery and GROW School for Oncology and Developmental Biology, Maastricht University Medical Center, Postbus 5800, P. Debyelaan, 6202 AZ, Maastricht, The Netherlands

[2]Department of Cranio-Maxillofacial Surgery and GROW School for Oncology and Developmental Biology, Maastricht University Medical Center, Postbus 5800, P. Debyelaan, 6202 AZ, Maastricht, The Netherlands

[3]Department of Oral and Maxillofacial Surgery, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

[4]Department of Experimental Molecular Imaging, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

[5]Department of Oral and Maxillofacial Surgery, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

[6]Department of Surgical Sciences, Health Sciences Center, Kuwait University, 13110, Safat, Kuwait

[7]Institute for Laboratory Animal Science and Experimental Surgery, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

关键词: Animal testing;    Cleft lip and palate;    Alveolar cleft;    Bone transplantation;    Grafting;    Tissue-engineering;    Dentin;    Rabbit;   

DOI  :  10.1186/s12967-017-1369-3

 received in 2017-10-28, accepted in 2017-12-15,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAlveolar cleft repair is performed via bone grafting procedure to restore the dental arch continuity. A suitable bone substitute materials should possess osteoinductive and osteoconductive properties, to promote new bone formation, along with a slowly resorbable scaffold that is subsequently replaced with functionally viable bone. Calcium phosphate biomaterials have long proved their efficacy as bone replacement materials. Dentin in several forms has also demonstrated its possibility to be used as bone graft replacement material in several studies. The purpose of this study was to evaluate bone regeneration pattern and quantify bone formation after grafting pre-established experimental alveolar clefts defects model in rabbits using composite xenogenic dentin and β-TCP in comparison to β-TCP alone.MethodsUnilateral alveolar cleft defects were created in 16 New Zealand rabbits according to previously described methodology. Alveolar clefts were allowed 8 weeks healing period. 8 defects were filled with β-TCP, whereas 8 defects filled with composite xenogenic dentin with β-TCP. Bone regeneration of the healed defects was compared at the 8 weeks after intervention. Quantification of bone formation was analyzed using micro-computed tomography (µCT) and histomorphometric analysis.ResultsµCT and histomorphometric analysis revealed that defects filled with composite dentin/β-TCP showed statistically higher bone volume fraction, bone mineral density and percentage residual graft volume when compared to β-TCP alone. An improved surgical handling of the composite dentin/β-TCP graft was also noted.ConclusionsComposite xenogenic dentin/β-TCP putty expresses enhanced bone regeneration compared to β-TCP alone in the reconstruction of rabbit alveolar clefts defects.

【 授权许可】

CC BY   
© The Author(s) 2017