Mycobacterium bovis infection at the interface between domestic and wild animals in Zambia

BMC Veterinary Research

Research Article

Chie Nakajima¹  Yukari Fukushima¹  Haruka Suzuki¹  Akihiro Ishii¹  Yasuhiko Suzuki²  Mudenda B Hang’ombe³  Aaron S Mweene³  Musso Munyeme³  Wigganson Matandiko⁴ 

[1] Hokkaido University Research Center for Zoonosis Control, Kita-20, Nishi-10, Kita-ku, 001-0020, Sapporo, Japan;Hokkaido University Research Center for Zoonosis Control, Kita-20, Nishi-10, Kita-ku, 001-0020, Sapporo, Japan;JST/JICA-SATREPS, 120-8666, Tokyo, Japan;School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia;Zambia Wildlife Authority, Private Bag 001, Chilanga, Zambia;

关键词: Bovine tuberculosis;    Cattle;    Mycobacterium bovis;    Strains;    Wildlife;    Kobus leche Kafuensis;   

DOI  :  10.1186/1746-6148-8-221

 received in 2012-06-19, accepted in 2012-10-26,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundIn Zambia, the presence of bovine tuberculosis in both wild and domestic animals has long been acknowledged and mutual transmission between them has been predicted without any direct evidence. Elucidation of the circulating Mycobacterium bovis strains at wild and domestic animals interphase area in Zambia, where bovine tuberculosis was diagnosed in wildlife seemed to be important.ResultsA PCR identified 15 and 37 M. bovis isolates from lechwe and cattle, respectively. Spoligotype analysis revealed that M. bovis strains from lechwe and cattle in Kafue basin clustered into a major node SB0120, where isolates outside the Kafue basin clustered into different nodes of SB0131 and SB0948. The comparatively higher variety of strains in cattle compared to lechwe elucidated by Mycobacterial Interspersed Repetitive Units–Variable Number Tandem Repeats analyses are consistent with cattle being the probable source of M. bovis in wild and domestic animals interphase area in Zambia.ConclusionsThese results provide strong evidence of M. bovis strains transfer between cattle and lechwe, with the latter having developed into a sylvatic reservoir host.

【 授权许可】

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© Hang'ombe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.