The early phase transcriptome of bovine monocyte-derived macrophages infected with Staphylococcus aureus in vitro

BMC Genomics

Research Article

Alison Downing¹  Richard Talbot¹  Guro Margrethe Boman²  Anna Monika Lewandowska-Sabat²  Ingrid Olsaker²  Anne Kristine Storset³ 

[1] ARK-Genomics, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, EH25 9PS, Roslin, Midlothian, UK;Department of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, P.O. Box 8146, Dep, NO-0033, Oslo, Norway;Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, NO-0033, Oslo, Norway;

关键词: Alternative activation;    Cattle;    Intracellular;    Macrophages;    Mastitis;    Microarray;    Staphylococcus aureus;   

DOI  :  10.1186/1471-2164-14-891

 received in 2013-05-10, accepted in 2013-12-03,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundIn the mammary gland, local recruitment and action of macrophages is a key immunological defence mechanism against infection. Macrophages are members of the innate immune system, serve as the first line of the defence against invading pathogens and are critical effectors and regulators of inflammation. We have examined the early phase response of bovine macrophages to infection with live Staphylococcus aureus. Genome-wide transcript profiling of blood monocyte-derived macrophages from six Norwegian Red heifers infected with live S. aureus for 2 and 6 hours in vitro was performed.ResultsAbout 420 of the 17 000 genes on the ARK-Genomics bovine cDNA array were differentially regulated at 6 hours post infection. Approximately 70% of the responding genes had a known identity (Entrez Gene ID) and were used in the identification of overrepresented pathways and biological functions in the dataset.Analysis of a subset of differentially regulated genes (List eQG) obtained by comparison with data from genome-wide association mapping in Norwegian Red cattle identified anti-inflammatory cytokines interleukin 4 and interleukin 13 as putative expression quantitative trait loci, suggesting that S. aureus infection triggers alternative activation of macrophages. Moreover, several classical activation pathways were found, mainly cellular immune response and cytokine signaling pathways, i.e. triggering receptor expressed on myeloid cells 1 (TREM1) and nucleotide-binding and oligomerization domain-like receptor (NLR) pathways. Tumor necrosis factor receptor superfamily member 5 (CD40 ligand) was identified as an upstream regulator which points toward CD40 likely acting as a co-stimulatory receptor during Toll-like receptor 2(TLR2)-mediated inflammatory response of bovine macrophages to S. aureus infection. Furthermore, peptidoglycan was identified as an upstream regulator in the List eQG, which indicates that this bacterial cell-wall component might be pivotal in macrophage intracellular bacterial recognition during early inflammation.ConclusionsHere we have shown that in vitro infection of bovine macrophages with live S. aureus induced both alternative and classical activation pathways. Alternative activation of macrophages may be a mechanism contributing to intracellular persistence of S. aureus in the course of inflammation such as during mastitis in dairy cattle.

【 授权许可】

Unknown   
© Lewandowska-Sabat et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.