Lipomatous metaplasia identified in rabbits with reperfused myocardial infarction by 3.0 T magnetic resonance imaging and histopathology

BMC Medical Imaging

Research Article

Stefan Janssens¹  Yi Xie²  Yicheng Ni³  Raymond Oyen³  Jan Bogaert³  Feng Chen⁴  Junjie Li⁴  Marlein Miranda Cona⁴  Huaijun Wang⁴  Yuanbo Feng⁴  Jie Yu⁴ 

[1] Department of Cardiovascular Diseases, University Hospitals, KU, Leuven, Belgium;Departments of Electronics and Information System (ELIS), Ghent University, Ghent, Belgium;Radiology Section, Department of Imaging and Pathology, University Hospitals, KU Leuven, Belgium;Radiology Section, Department of Imaging and Pathology, University Hospitals, KU Leuven, Belgium;Molecular Small Animal Imaging Center (MoSAIC), Biomedical Sciences Group, University Hospitals, KU Leuven, Belgium;

关键词: Acute myocardial infarction;    Animal model;    Chronic ischemia;    Cardiovascular magnetic resonance imaging;    Lipomatous metaplasia;   

DOI  :  10.1186/1471-2342-13-18

 received in 2012-10-28, accepted in 2013-05-21,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundCardiac lipomatous metaplasia (LM) occurs in patients with chronic ischemic heart disease and heart failure with unclear mechanisms. We studied coronary occlusion/reperfusion-induced myocardial infarction (MI) in rabbits during a 9-months follow-up using 3.0 T magnetic resonance scanner, and confirmed the presence of MI in acute phase and LM in chronic phase using histopathology.MethodsMI was surgically induced in 10 rabbits by 90-min coronary artery occlusion and reperfusion. Forty-eight hours later, multiparametric cardiac magnetic resonance imaging (cMRI) was performed at a 3.0 T clinical scanner for MI diagnosis and cardiac function analysis. Afterwards, seven rabbits were scarified for histochemical staining with triphenyltetrazolium chloride (TTC), and hematoxylin-eosin (HE), and 3 were scanned with cMRI at 2 days, 2 weeks, 2 months and 9 months for longitudinal observations of morphological and functional changes, and the fate of the animals. Post-mortem TTC, HE and Masson's trichrome (MTC) were studied for chronic stage of MI.ResultsThe size of acute MI correlated well between cMRI and TTC staining (r2=0.83). Global cardiac morphology-function analysis showed significant correlation between increasing acute MI size and decreasing ejection fraction (p<0.001). During 9 months, cMRI documented evolving morphological and functional changes from acute MI to chronic scar transformation and fat deposition with a definite diagnosis of LM established by histopathology.ConclusionsAcute MI and chronic LM were induced in rabbits and monitored with 3.0 T MRI. Studies on this platform may help investigate the mechanisms and therapeutic interventions for LM.

【 授权许可】

Unknown   
© Feng et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.