The ZnO-FET Biosensor for Cardiac Troponin I

Malaysian Technical Universities Conference on Engineering and Technology 2017

Fathil, M.F.M.^1 ; Arshad, M.K.Md.^1,2 ; Nuzaihan, M.N.M.^1 ; Gopinath, Subash C.B.^1,3 ; Ruslinda, A.R.^1 ; Hashim, U.^1,2

Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar, Perlis

01000, Malaysia^1

School of Microelectronic Engineering, Universiti Malaysia Perlis, Perlis, Arau

02600, Malaysia^2

School of Bioprocess Engineering, Universiti Malaysia Perlis, Perlis, Arau

02600, Malaysia^3

关键词: 3-aminopropyltriethoxysilane;    Acute myocardial infarction;    Cardiac troponin I;    Conventional lithography;    Electron beam evaporators;    Limit of detection;    N-type zinc oxides;    Sensitivity increase;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/318/1/012031/pdf
DOI  :  10.1088/1757-899X/318/1/012031

来源: IOP

【 摘 要 】

This paper investigates the influence of substrate-gate coupling on the ZnO-FET biosensor's sensitivity for detection of cardiac troponin I (cTnI), a 'gold standard' biomarker for acute myocardial infarction (AMI). The FET-based device with introduction of substrate-gate coupling on p-type silicon-on-insulator (SOI) substrate is fabricated using conventional lithography processes. An n-type zinc oxide (ZnO) thin film deposited via electron-beam evaporator is used as transducer for bridging the source and drain regions. Surface modifications via functionalization with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA) as chemical linkers, followed by immobilization of cTnI monoclonal antibody (MAb-cTnI) as bio-receptor on the ZnO thin film allow different concentration of cTnI detection with high selectivity. The device's sensitivity increases up to 9 %•(g/ml)-1with the increase of the substrate-gate voltage (VSG) up to -10 V at very low limit of detection (LOD) down to 1.6 fg/ml.