Malaysian Technical Universities Conference on Engineering and Technology 2017 | |
The ZnO-FET Biosensor for Cardiac Troponin I | |
Fathil, M.F.M.^1 ; Arshad, M.K.Md.^1,2 ; Nuzaihan, M.N.M.^1 ; Gopinath, Subash C.B.^1,3 ; Ruslinda, A.R.^1 ; Hashim, U.^1,2 | |
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar, Perlis | |
01000, Malaysia^1 | |
School of Microelectronic Engineering, Universiti Malaysia Perlis, Perlis, Arau | |
02600, Malaysia^2 | |
School of Bioprocess Engineering, Universiti Malaysia Perlis, Perlis, Arau | |
02600, Malaysia^3 | |
关键词: 3-aminopropyltriethoxysilane; Acute myocardial infarction; Cardiac troponin I; Conventional lithography; Electron beam evaporators; Limit of detection; N-type zinc oxides; Sensitivity increase; | |
Others : https://iopscience.iop.org/article/10.1088/1757-899X/318/1/012031/pdf DOI : 10.1088/1757-899X/318/1/012031 |
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来源: IOP | |
【 摘 要 】
This paper investigates the influence of substrate-gate coupling on the ZnO-FET biosensor's sensitivity for detection of cardiac troponin I (cTnI), a 'gold standard' biomarker for acute myocardial infarction (AMI). The FET-based device with introduction of substrate-gate coupling on p-type silicon-on-insulator (SOI) substrate is fabricated using conventional lithography processes. An n-type zinc oxide (ZnO) thin film deposited via electron-beam evaporator is used as transducer for bridging the source and drain regions. Surface modifications via functionalization with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA) as chemical linkers, followed by immobilization of cTnI monoclonal antibody (MAb-cTnI) as bio-receptor on the ZnO thin film allow different concentration of cTnI detection with high selectivity. The device's sensitivity increases up to 9 %•(g/ml)-1with the increase of the substrate-gate voltage (VSG) up to -10 V at very low limit of detection (LOD) down to 1.6 fg/ml.
【 预 览 】
Files | Size | Format | View |
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The ZnO-FET Biosensor for Cardiac Troponin I | 1381KB | download |