Lipomatous metaplasia identified in rabbits with reperfused myocardial infarction by 3.0 T magnetic resonance imaging and histopathology

BMC Medical Imaging

Yicheng Ni⁴  Raymond Oyen⁴  Stefan Janssens²  Jan Bogaert⁴  Junjie Li³  Jie Yu³  Marlein Miranda Cona³  Huaijun Wang³  Yi Xie¹  Feng Chen³  Yuanbo Feng³ 

[1] Departments of Electronics and Information System (ELIS), Ghent University, Ghent, Belgium;Department of Cardiovascular Diseases, University Hospitals, KU, Leuven, Belgium;Molecular Small Animal Imaging Center (MoSAIC), Biomedical Sciences Group, University Hospitals, KU Leuven, Belgium;Radiology Section, Department of Imaging and Pathology, University Hospitals, KU Leuven, Belgium

关键词: Lipomatous metaplasia;    Cardiovascular magnetic resonance imaging;    Chronic ischemia;    Animal model;    Acute myocardial infarction;   

Others  :  1090979
DOI  :  10.1186/1471-2342-13-18

 received in 2012-10-28, accepted in 2013-05-21,  发布年份 2013

【 摘 要 】

Background

Cardiac lipomatous metaplasia (LM) occurs in patients with chronic ischemic heart disease and heart failure with unclear mechanisms. We studied coronary occlusion/reperfusion-induced myocardial infarction (MI) in rabbits during a 9-months follow-up using 3.0 T magnetic resonance scanner, and confirmed the presence of MI in acute phase and LM in chronic phase using histopathology.

Methods

MI was surgically induced in 10 rabbits by 90-min coronary artery occlusion and reperfusion. Forty-eight hours later, multiparametric cardiac magnetic resonance imaging (cMRI) was performed at a 3.0 T clinical scanner for MI diagnosis and cardiac function analysis. Afterwards, seven rabbits were scarified for histochemical staining with triphenyltetrazolium chloride (TTC), and hematoxylin-eosin (HE), and 3 were scanned with cMRI at 2 days, 2 weeks, 2 months and 9 months for longitudinal observations of morphological and functional changes, and the fate of the animals. Post-mortem TTC, HE and Masson's trichrome (MTC) were studied for chronic stage of MI.

Results

The size of acute MI correlated well between cMRI and TTC staining (r²=0.83). Global cardiac morphology-function analysis showed significant correlation between increasing acute MI size and decreasing ejection fraction (p<0.001). During 9 months, cMRI documented evolving morphological and functional changes from acute MI to chronic scar transformation and fat deposition with a definite diagnosis of LM established by histopathology.

Conclusions

Acute MI and chronic LM were induced in rabbits and monitored with 3.0 T MRI. Studies on this platform may help investigate the mechanisms and therapeutic interventions for LM.

【 授权许可】

   
2013 Feng et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Fonge H, Vunckx K, Wang H, Feng Y, Mortelmans L, Nuyts J, et al.: Non-invasive detection and quantification of acute myocardial infarction in rabbits using mono-[123I]iodohypericin microSPECT. Eur Heart J 2008, 29(2):260-269.
• [2]Feng Y, Xie Y, Wang H, Chen F, Ye Y, Jin L, et al.: A modified rabbit model of reperfused myocardial infarction for cardiac MR imaging research. Int J Cardiovasc Imaging 2009, 25(3):289-298.
• [3]Feng Y, Xie Y, Wang H, Chen F, Marchal G, Ni Y: Animal models of ischemic heart disease for cardiac MR imaging research. International Journal of Modelling, Identification and Control 2010, 9:288-310.
• [4]Greenman RL, Shirosky JE, Mulkern RV, Rofsky NM: Double inversion black-blood fast spin-echo imaging of the human heart: a comparison between 1.5T and 3.0T. J Magn Reson Imaging 2003, 17(6):648-655.
• [5]Hinton DP, Wald LL, Pitts J, Schmitt F: Comparison of cardiac MRI on 1.5 and 3.0 Tesla clinical whole body systems. Invest Radiol 2003, 38(7):436-442.
• [6]Kimura F, Matsuo Y, Nakajima T, Nishikawa T, Kawamura S, Sannohe S, et al.: Myocardial fat at cardiac imaging: how can we differentiate pathologic from physiologic fatty infiltration? Radiographics 2010, 30(1):1587-1602.
• [7]van den Borne SWM, Diez J, Blankesteijn WM, Verjans J, Hofstra L, Narula J: Myocardial remodeling after infarction: the role of myofibroblasts. Nat Rev Cardiol 2010, 7(1):30-37.
• [8]Lucke C, Schindler K, Lehmkuhl L, Grothoff M, Eitel I, Schuler G, et al.: Prevalence and functional impact of lipomatous metaplasia in scar tissue following myocardial infarction evaluated by MRI. Eur Radiol 2010, 20(9):2074-2083.
• [9]Baroldi G, Silver MD, De Maria R, Parodi O, Pellegrini A: Lipomatous metaplasia in left ventricular scar. Can J Cardiol 1997, 13(1):65-71.
• [10]Agudelo CF, Fictum P, Skoric M, Kazbundova K, Svoboda M, Scheer P: Unusual massive fatty infiltration of the heart in a British cat: a case report. Vet Med 2011, 56(3):145-147.
• [11]Choi SH, Kang JW, Kim ST, Lee BH, Chun EJ, Schuleri KH, et al.: Investigation of T2-weighted signal intensity of infarcted myocardium and its correlation with delayed enhancement magnetic resonance imaging in a porcine model with reperfused acute myocardial infarction. Int J Cardiovasc Imaging 2009, 25(Suppl 1):111-119.
• [12]Saeed M, Lee RJ, Weber O, Do L, Martin A, Ursell P, et al.: Scarred myocardium imposes additional burden on remote viable myocardium despite a reduction in the extent of area with late contrast MR enhancement. Eur Radiol 2006, 16(4):827-836.
• [13]Aquaro GD, Nucifora G, Pederzoli L, Strata E, De Marchi D, Todiere G, et al.: Fat in left ventricular myocardium assessed by steady-state free precession pulse sequences. Int J Cardiovasc Imaging 2012, 28(4):813-821.
• [14]Okayama S, Uemura S, Sugimoto H, Enomoto S, Onoue K, Omori S, et al.: Dual gradient-echo in-phase and opposed-phase magnetic resonance imaging to evaluate lipomatous metaplasia in patients with old myocardial infarction. Magn Reson Med Sci 2010, 9(2):85-89.
• [15]Okayama S, Uemura S, Watanabe M, Morikawa Y, Onoue K, Soeda T, et al.: Novel application of black-blood echo-planar imaging to the assessment of myocardial infarction. Heart Vessels 2010, 25(2):104-112.
• [16]Arai AE: Magnetic resonance imaging for area at risk, myocardial infarction, and myocardial salvage. J Cardiovasc Pharmacol Ther 2011, 16(3–4):313-320.
• [17]Valle-Munoz A, Estornell-Erill J, Corbi-Pascual M, Ridocci-Soriano F: Lipomatous metaplasia. Two chronic infarcts in the same patient detected by cardiac magnetic resonance. Rev Esp Cardiol 2009, 62(7):830-831.
• [18]Arnold JR, Karamitsos TD, Pegg TJ, Francis JM, Neubauer S: Left ventricular lipomatous metaplasia following myocardial infarction. Int J Cardiol 2009, 137(1):e11-e12.
• [19]Goldfarb JW, Roth M, Han J: Myocardial fat deposition after left ventricular myocardial infarction: assessment by using MR water-fat separation imaging. Radiology 2009, 253(1):65-73.
• [20]Wheeler LD, Wood AM: Re: CT imaging features and frequency of left ventricular myocardial fat in patients with CT findings of chronic left ventricular myocardial infarction. Clin Radiol 2008, 63(10):1184-1185. author reply 1185
• [21]Zafar HM, Litt HI, Torigian DA: CT imaging features and frequency of left ventricular myocardial fat in patients with CT findings of chronic left ventricular myocardial infarction. Clin Radiol 2008, 63(3):256-262.
• [22]Ichikawa Y, Kitagawa K, Chino S, Ishida M, Matsuoka K, Tanigawa T, et al.: Adipose tissue detected by multislice computed tomography in patients after myocardial infarction. JACC Cardiovasc Imaging 2009, 2(5):548-555.
• [23]Lehmann GM, Woeller CF, Pollock SJ, O’Loughlin CW, Gupta S, Feldon SE, et al.: Novel anti-adipogenic activity produced by human fibroblasts. Am J Physiol Cell Physiol 2010, 299(1):c672-c681.
• [24]Su L, Siegel JE, Fishbein MC: Adipose tissue in myocardial infarction. Cardiovasc Pathol 2004, 13(2):98-102.
• [25]Schmitt M, Samani N, McCann G: Images in cardiovascular medicine. Lipomatous metaplasia in ischemic cardiomyopathy: a common but unappreciated entity. Circulation 2007, 116(1):e5-e6.
• [26]McKeag NA, Harbinson MT, McKeown PP, Roberts MJ: Lipomatous metaplasia within an old anterior myocardial infarction. Qjm-an International Journal of Medicine 2011, 104(12):1101-1102.
• [27]van der Laarse A, van der Wall EE: Rabbit models: ideal for imaging purposes? Int J Cardiovasc Imaging 2009, 25(3):299-301.