In vivo expression of innate immunity markers in patients with mycobacterium tuberculosis infection

BMC Infectious Diseases

Research Article

Nikolaos Poulakis¹  Pantelis Constantoulakis²  Eftihia Filiou²  Aggeliki Hamhougia³  Simona Karabela⁴  Nikoletta Rovina⁵  George Chras⁵  Adamandia Sotiriou⁵  Charis Roussos⁵ 

[1] 1st Pulmonary Department, "Sotiria" District Chest Diseases Hospital, Athens, Greece;Locus Medicus, Molecular Pathology and Genetics Dept, Athens, Greece;Mathematics Dept, University of Athens, Athens, Greece;National Center of Tuberculosis, Microbiology Dpt, "Sotiria" District Chest Diseases, Athens, Greece;National and Kapodistrian University of Athens, Pulmonary Department, "Sotiria" District Chest Diseases Hospital, Athens, Greece;

关键词: Tuberculosis;    Tuberculosis Infection;    Mycobacterial Antigen;    Pathogenic Mycobacterium;    Coronin;   

DOI  :  10.1186/1471-2334-10-243

 received in 2010-02-07, accepted in 2010-08-18,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundToll-like receptors (TLRs), Coronin-1 and Sp110 are essential factors for the containment of Mycobacterium tuberculosis infection. The purpose of this study was to investigate the in vivo expression of these molecules at different stages of the infection and uncover possible relationships between these markers and the state of the disease.MethodsTwenty-two patients with active tuberculosis, 15 close contacts of subjects with latent disease, 17 close contacts of subjects negative for mycobacterium antigens and 10 healthy, unrelated to patients, subjects were studied. Quantitative mRNA expression of Coronin-1, Sp110, TLRs-1,-2,-4 and -6 was analysed in total blood cells vs an endogenous house-keeping gene.ResultsThe mRNA expression of Coronin-1, Sp110 and TLR-2 was significantly higher in patients with active tuberculosis and subjects with latent disease compared to the uninfected ones. Positive linear correlation for the expression of those factors was only found in the infected populations.ConclusionsOur results suggest that the up-regulation of Coronin-1 and Sp110, through a pathway that also includes TLR-2 up-regulation may be involved in the process of tuberculous infection in humans. However, further studies are needed, in order to elucidate whether the selective upregulation of these factors in the infected patients could serve as a specific molecular marker of tuberculosis.

【 授权许可】

Unknown   
© Constantoulakis et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.