Passive administration of purified secretory IgA from human colostrum induces protection against Mycobacterium tuberculosis in a murine model of progressive pulmonary infection

BMC Immunology

Proceedings

María E Sarmiento¹  Reinier Borrero¹  Armando Acosta¹  Gustavo Falero-Díaz¹  Oscar Otero¹  Alina Puig¹  Alicia Aguilar¹  Nadine Alvarez¹  Yanely Tirado¹  Frank Camacho¹  Armando Cádiz²  Rogelio Hernández-Pando³  Axel Cervantes³  Cesar Rivas³  Mohd Nor Norazmi⁴ 

[1] Department of Molecular Biology. Finlay Institute, Center of Research – Producction of Vaccines, 11600, Ave. 27 No. 19805, La Lisa. Ciudad de la Habana, 16017, APCP, Cuba;Enterprise of Production of Serum and Hemoderivates “Adalberto Pesant González", Ave 51 No.33 235 km 19 medio ½. Arroyo Arenas, La Lisa. Ciudad de la Habana, 13400, CP, Cuba;Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition “Salvador Zubiràn" D.F, 14 000, CP, Mexico;School of Health Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Malaysia;Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 16150, Kubang Kerian, Malaysia;

关键词: Tuberculosis;    Mycobacterial Infection;    Protective Capacity;    Secretor Component;    Mycobacterial Antigen;   

DOI  :  10.1186/1471-2172-14-S1-S3

来源: Springer

【 摘 要 】

BackgroundImmunoglobulin A is the most abundant isotype in secretions from mucosal surfaces of the gastrointestinal, respiratory and genitourinary tracts and in external secretions such as colostrum, breast milk, tears and saliva. The high concentration of human secretory IgA (hsIgA) in human colostrum strongly suggests that it should play an important role in the passive immune protection against gastrointestinal and respiratory infections.Materials and methodsHuman secretory IgA was purified from colostrum. The reactivity of hsIgA against mycobacterial antigens and its protective capacity against mycobacterial infection was evaluated.ResultsThe passive administration of hsIgA reduces the pneumonic area before challenge with M. tuberculosis. The intratracheal administration of M. tuberculosis preincubated with hsIgA to mice greatly reduced the bacterial load in the lungs and diminished lung tissue injury.ConclusionsHsIgA purified from colostrum protects against M. tuberculosis infection in an experimental mouse model.

【 授权许可】

Unknown   
© Alvarez et al; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.