Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses

Lipids in Health and Disease

Research

Jesús Martínez-Reding¹  Julio Sandoval-Zárate²  Valentín Herrera-Alarcón³  Nydia Ávila-Vanzzini⁴  Jesús Vargas-Barrón⁵  Carlos Martínez-Sánchez⁶  María Luna-Luna⁷  David Cruz-Robles⁷  José Manuel Fragoso⁸  Elizabeth Carreón-Torres⁸  Óscar Pérez-Méndez⁸  Gilberto Vargas-Alarcón⁸  Armando Roberto Tovar-Palacio⁹  Sergio Criales-Vera^1,10 

[1] Department of Adult Cardiology, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Department of Cardiopulmonary, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Study Group of Atherosclerosis, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Department of Cardiothoracic Surgery, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Department of Echocardiography, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Department of Echocardiography, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Study Group of Atherosclerosis, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Department of Emergency, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Study Group of Atherosclerosis, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano 1, Sección XVI, 14080, México City, D.F., Mexico;Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano 1, Sección XVI, 14080, México City, D.F., Mexico;Study Group of Atherosclerosis, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Department of Physiology of Nutrition, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”, México City, Mexico;Department of Radiology, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;Study Group of Atherosclerosis, Instituto Nacional de Cardiología “Ignacio Chávez”, México City, Mexico;

关键词: Adipose tissue;    High-density lipoprotein;    Osteopontin;    Osteonectin;    Osteoprotegerin;   

DOI  :  10.1186/s12944-017-0550-2

 received in 2017-06-28, accepted in 2017-08-14,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundPrevious studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated by high-density lipoprotein (HDL) subclasses.MethodsTo explore this possibility, we determined the mRNA expression by qPCR of a pro-calcifying gene (osteopontin (OPN)), and two anti-calcifying genes (osteoprotegerin (OPG) and osteonectin (ON)), in biopsies of EAT obtained from 15 patients with coronary artery disease (CAD) determined by angiography, and 15 patients with diagnostic of aortic valve stenosis but without CAD as control group. We determined the distribution and composition of HDL subclasses by electrophoresis and their statistical relationship with the gene expression in EAT.ResultsEAT from CAD patients showed a higher expression level of OPN and OPG than control group, whereas ON expression was similar between groups. Large HDL subclasses were cholesterol-poor in CAD patients as estimated by the cholesterol-to-phospholipid ratio. A linear regression model showed an independent association of OPN expression with HDL3a-cholesterol, and OPG expression with the relative proportion of HDL3b protein. Logistic analysis determined that OPN expression was positively associated with the presence of atherosclerotic plaqueConclusionOPN, ON, and OPG genes are transcribed in EAT; to the exception of ON, the level of expression was different in CAD patients and control group, and correlated with some HDL subclasses, suggesting a new role of these lipoproteins.

【 授权许可】

CC BY   
© The Author(s). 2017