Quantitative Analysis of Osteonectin mRNA in Thyroid Carcinomas

Endocrine Journal

KANJI KUMA³  HIROSHI YOSHIDA³  NOBUYASU HAYASHI¹  AKIRA MIYAUCHI³  YUKIKO HASEGAWA²  SHOJI NAKAMORI¹  FUMIO MATSUZUKA³  NOBUYUKI AMINO²  TORU TAKANO² 

[1] Department of Surgery and Clinical Oncology, Osaka University Medical School;Department of Laboratory Medicine, Osaka University Medical School;Kuma Hospital

关键词: Thyroid carcinoma;    Reverse transcription-PCR;    Fine needle aspiration biopsy;    Osteonectin;   

DOI  :  10.1507/endocrj.49.511

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(30)Cited-By(10)Our recent study of the gene expression profile in thyroid carcinoma showed an overexpression of osteonectin mRNA, an extracellular matrix protein, in an anaplastic carcinoma. To confirm this, we measured the expression levels of osteonectin mRNA in 84 thyroid normal and tumor tissues, including five anaplastic carcinomas by realtime quantitative reverse-transcription PCR. Increased expression of osteonectin mRNA was observed in anaplastic carcinoma tissue. However, in five anaplastic carcinoma cell lines, no increase was observed in the expression levels of osteonectin mRNA. These findings suggest the possibility that increased expression of osteonectin mRNA in anaplastic carcinoma tissue may be due to its overexpression in stromal cells, but not in anaplastic carcinoma cells.

【 授权许可】

Unknown