Preoperative Diagnosis of Thyroid Carcinomas by Aspiration Biopsy-Reverse Transcription-Polymerase Chain Reaction (ABRP)

Endocrine Journal

KYOTARO YOSHIDA²  HISATO TADA¹  HIDEO YOSHIMURA²  NOBUYUKI AMINO¹  EIICHI SHIBA³  REIKO DOI²  TORU TAKANO¹ 

[1] Departments of Laboratory Medicine, Osaka University Medical School;Department of Clinical Pathology, Osaka University Medical School;Department of Surgical Oncology, Osaka University Medical School

关键词: Thyroid carcinoma;    Reverse transcription-PCR;    Fine needle aspiration biopsy;    Oncofetal fibronectin;   

DOI  :  10.1507/endocrj.47.271

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(12)Cited-By(9)A preoperative molecular-based diagnostic technique for thyroid papillary and anaplastic carcinomas was recently developed to detect oncofetal fibronectin (onfFN) messenger RNA (mRNA) in fine needle aspiration biopsy (FNAB) by means of reverse transcription-polymerase chain reaction (RT-PCR). We report two cases of thyroid tumors, in which cytological diagnosis was not successful but in which RT-PCR analysis provided information that helped to identify the biological features of the tumors. One case could not be diagnosed by aspiration biopsy cytology (ABC) because of poor fixation; however, RT-PCR did detect the expression of onfFN mRNA, which suggested the existence of papillary carcinoma cells. After surgery, this tumor was diagnosed as a papillary carcinoma by histological examination. The other case was diagnosed as a papillary carcinoma by ABC, but onfFN mRNA was not detected in the FNAB by RT-PCR. The neoplasm was diagnosed as a follicular tumor by histological examination. These cases suggest the benefits of combining both genetic and cytological approaches to the examination of FNAB.

【 授权许可】

Unknown