Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria

Malaria Journal

Research

Rashidul Haque¹  Mohammad Shafiul Alam¹  Wasif A Khan¹  Abu Naser Mohon¹  Rubayet Elahi² 

[1] International Centre for Diarrhoeal Disease Research Bangladesh (icddr,b), Dhaka, Bangladesh;International Centre for Diarrhoeal Disease Research Bangladesh (icddr,b), Dhaka, Bangladesh;Department of Neurobiology and Developmental Sciences, University of Arkansas for Medical Sciences (UAMS), Little Rock, AR, USA;

关键词: Malaria;    Rapid Diagnostic Test;    Parasite Density;    Vivax Malaria;    Expert Microscopy;   

DOI  :  10.1186/1475-2875-12-378

 received in 2013-09-02, accepted in 2013-10-28,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe rapid diagnostic test (RDT) has been adopted in contemporary malaria control and management programmes around the world as it represents a fast and apt alternative for malaria diagnosis in a resource-limited setting. This study assessed the performance of a HRP-2/pLDH based RDT (Parascreen® Pan/Pf) in a laboratory setting utilizing clinical samples obtained from the field.MethodsWhole blood samples were obtained from febrile patients referred for malaria diagnosis by clinicians from two different Upazila Health Complexes (UHCs) located near the Bangladesh-India and Bangladesh-Myanmar border where malaria is endemic. RDT was performed on archived samples and sensitivity and specificity evaluated with expert microscopy (EM) and quantitative PCR (qPCR).ResultsA total of 327 clinical samples were made available for the study, of which 153 were Plasmodium falciparum- positive and 54 were Plasmodium vivax- positive. In comparison with EM, for P. falciparum malaria, the RDT had sensitivity: 96.0% (95% CI, 91.2-98.3) and specificity: 98.2% (95% CI, 94.6-99.5) and for P. vivax, sensitivity: 90.7% (95% CI, 78.9-96.5) and specificity: 98.9% (95% CI, 96.5-99.7). Comparison with qPCR showed, for P. falciparum malaria, sensitivity: 95.4% (95% CI, 90.5-98.0) and specificity: 98.8% (95% CI, 95.4-99.7) and for P. vivax malaria, sensitivity: 89.0% (95% CI,77.0-95.4) and specificity: 98.8% (95% CI, 96.5-99.7). Sensitivity varied according to different parasitaemia for falciparum and vivax malaria diagnosis.ConclusionParascreen® Pan/Pf Rapid test for malaria showed acceptable sensitivity and specificity in border belt endemic areas of Bangladesh when compared with EM and qPCR.

【 授权许可】

Unknown   
© Elahi et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.