Increased interleukin-10 and interferon-γ levels in Plasmodium vivax malaria suggest a reciprocal regulation which is not altered by IL-10 gene promoter polymorphism

Malaria Journal

Research

João S Silva¹  Tassia F Gomes²  Tiago S Medina²  Sheyla PT Costa²  Maristela G Cunha²  Antonio CR Vallinoto³  Ana M Ventura⁴  Maria D Oliveira⁴  José M Souza⁴  Marinete M Póvoa⁵ 

[1] Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, CEP 14040-030, Ribeirão Preto, São Paulo, Brazil;Laboratório de Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, CEP 66075-970, Belém, Pará, Brazil;Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, CEP 66075-970, Belém, Pará, Brazil;Programa de Ensaios Clínicos em Malária, Instituto Evandro Chagas/SVS/MS, CEP 67030-000, Ananindeua, Pará, Brazil;Seção de Parasitologia, Instituto Evandro Chagas/SVS/MS, CEP 67030-000, Ananindeua, Pará, Brazil;

关键词: Malaria;    Parasite Density;    Vivax Malaria;    Vivax Infection;    Thick Blood Smear;   

DOI  :  10.1186/1475-2875-10-264

 received in 2011-06-28, accepted in 2011-09-14,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundIn human malaria, the naturally-acquired immune response can result in either the elimination of the parasite or a persistent response mediated by cytokines that leads to immunopathology. The cytokines are responsible for all the symptoms, pathological alterations and the outcome of the infection depends on the reciprocal regulation of the pro and anti-inflammatory cytokines. IL-10 and IFN-gamma are able to mediate this process and their production can be affected by single nucleotide polymorphisms (SNPs) on gene of these cytokines. In this study, the relationship between cytokine IL-10/IFN-gamma levels, parasitaemia, and their gene polymorphisms was examined and the participation of pro-inflammatory and regulatory balance during a natural immune response in Plasmodium vivax-infected individuals was observed.MethodsThe serum levels of the cytokines IL-4, IL-12, IFN-gamma and IL-10 from 132 patients were evaluated by indirect enzyme-linked immunosorbent assays (ELISA). The polymorphism at position +874 of the IFN-gamma gene was identified by allele-specific polymerase chain reaction (ASO-PCR) method, and the polymorphism at position -1082 of the IL-10 gene was analysed by PCR-RFLP (PCR-Restriction Fragment Length Polymorphism).ResultsThe levels of a pro- (IFN-gamma) and an anti-inflammatory cytokine (IL-10) were significantly higher in P. vivax-infected individuals as compared to healthy controls. The IFN-gamma levels in primoinfected patients were significantly higher than in patients who had suffered only one and more than one previous episode. The mutant alleles of both IFN-gamma and IL-10 genes were more frequent than the wild allele. In the case of the IFNG+874 polymorphism (IFN-gamma) the frequencies of the mutant (A) and wild (T) alleles were 70.13% and 29.87%, respectively. Similar frequencies were recorded in IL-10-1082, with the mutant (A) allele returning a frequency of 70.78%, and the wild (G) allele a frequency of 29.22%. The frequencies of the alleles associated with reduced production of both IFN-gamma and IL-10 were high, but this effect was only observed in the production of IFN-gamma.ConclusionsThis study has shown evidence of reciprocal regulation of the levels of IL-10 and IFN-gamma cytokines in P. vivax malaria, which is not altered by the presence of polymorphism in the IL-10 gene.

【 授权许可】

Unknown   
© Medina et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.