Antifungal activity of schinol and a new biphenyl compound isolated from Schinus terebinthifolius against the pathogenic fungus Paracoccidioides brasiliensis

Annals of Clinical Microbiology and Antimicrobials

Research

Patricia S Cisalpino¹  Betania B Cota²  Tânia MA Alves²  Ezequias P Siqueira²  Carlos L Zani²  Nívea P Sá²  Luciana ARS Lima³  Susana Johann⁴ 

[1] Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;Laboratório de Química de Produtos Naturais, Centro de Pesquisas René Rachou, Belo Horizonte, MG, Brazil;Laboratório de Química de Produtos Naturais, Centro de Pesquisas René Rachou, Belo Horizonte, MG, Brazil;Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del-Rei, Divinópolis, MG, Brazil;Laboratório de Química de Produtos Naturais, Centro de Pesquisas René Rachou, Belo Horizonte, MG, Brazil;Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;

关键词: Quercetin;    Sorbitol;    Antifungal Activity;    Itraconazole;    Minimal Inhibitory Concentration;   

DOI  :  10.1186/1476-0711-9-30

 received in 2010-08-05, accepted in 2010-10-12,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe aim of this study was to isolate and identify the antifungal compounds from the extracts of Schinus terebinthifolius (Anacardiaceae) against clinical isolates of the pathogenic fungus Paracoccidioides brasiliensis.MethodsThe hexane and dichlomethane fractions from leaves and stems of S. terebinthifolius were fractionated using several chromatography techniques to afford four compounds.ResultsThe compounds isolated from S. terebinthifolius were identified as schinol (1), a new biphenyl compound, namely, 4'-ethyl-4-methyl-2,2',6,6'-tetrahydroxy[1,1'-biphenyl]-4,4'-dicarboxylate (2), quercetin (3), and kaempferol (4). Compounds 1 and 2 were active against different strains of P. brasiliensis, showing a minimal inhibitory concentration value against the isolate Pb B339 of 15.6 μg/ml. The isolate Pb 1578 was more sensitive to compound 1 with a MIC value of 7.5 μg/ml. Schinol presented synergistic effect only when combined with itraconazole. The compounds isolated from S. terebinthifolius were not able to inhibit cell wall synthesis or assembly using the sorbitol assay.ConclusionThis work reveals for the first time the occurrence of compound 2 and discloses activity of compounds 1 and 2 against several clinical isolates of P. brasiliensi s. These results justify further studies to clarify the mechanisms of action of these compounds.

【 授权许可】

CC BY   
© Johann et al; licensee BioMed Central Ltd. 2010