Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry

BMC Microbiology

Research Article

John R. Barr¹  Jakub Baudys¹  Suzanne R. Kalb¹ 

[1] Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, NE, 30341, Atlanta, GA, USA;

关键词: Botulinum neurotoxin;    Botulism;    Mass spectrometry;   

DOI  :  10.1186/s12866-015-0567-5

 received in 2015-04-21, accepted in 2015-10-15,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe disease botulism is caused by intoxication with botulinum neurotoxins (BoNTs), extremely toxic proteins which cause paralysis. This neurotoxin is produced by some members of the Clostridium botulinum and closely related species, and is produced as a protein complex consisting of the neurotoxin and neurotoxin-associated proteins (NAPs). There are seven known serotypes of BoNT, A-G, and the composition of the NAPs can differ between these serotypes. It was previously published that the BoNT/G complex consisted of BoNT/G, nontoxic-nonhemagglutinin (NTNH), Hemagglutinin 70 (HA-70), and HA-17, but that HA-33, a component of the protein complex of other serotypes of BoNT, was not found.MethodsComponents of the BoNT/G complex were first separated by SDS-PAGE, and bands corresponding to components of the complex were digested and analyzed by LC-MS/MS.ResultsGel bands were identified with sequence coverages of 91 % for BoNT/G, 91 % for NTNH, 89 % for HA-70, and 88 % for HA-17. Notably, one gel band was also clearly identified as HA-33 with 93 % sequence coverage.ConclusionsThe BoNT/G complex consists of BoNT/G, NTNH, HA-70, HA-17, and HA-33. These proteins form the progenitor form of BoNT/G, similar to all other HA positive progenitor toxin complexes.

【 授权许可】

CC BY   
© Kalb et al. 2015