Rapid Generation of a Nanocrystal-Labeled Peptide Library for Specific Identification of the Bacterium Clostrium Botulinum.

Cohen, B. I. ; Hooper, E. B. ; Cohen, R. H. ; Hill, D. N. ; McLean, H. S. ; Wood, R. D. ; Woodruff, S.

Technical Information Center Oak Ridge Tennessee

关键词: Crystal structures;    Bacterium clostridium;    Neurotoxins;    Botulism;    Peptides;   

RP-ID  :  DE200515011397

学科分类：工程和技术（综合）

美国|英语

来源: National Technical Reports Library

【 摘 要 】

Several peptide libraries containing up to 2 million unique peptide ligands have been synthesized. The peptides are attached onto a 80 micron resin and the length of these peptide ligands ranges from 5 to 9 amino acid residues. Using a novel calorimetric assay, the libraries were screened for binding to the ganglioside-binding domain of Clostridium Tetanus Toxin, a structural similar analog of the Clostridium Botulinum toxin. Several binding peptide sequences were identified, in which the detailed binding kinetics are currently underway using the Surface Plasmon Resonance (SPR) technique.