Differential proteins among normal cervix cells and cervical cancer cells with HPV-16 infection, through mass spectrometry-based Proteomics (2D-DIGE) in women from Southern México

Proteome Science

Research

Marco Antonio Jiménez-López¹  Berenice Illades-Aguiar²  Olga Lilia Garibay-Cerdenares²  Pavel Sierra-Martínez³  Idanya Serafín-Higuera³  Eugenia Flores-Alfaro³  Luz del Carmen Alarcón-Romero⁴ 

[1] Instituto Estatal de Cancerología “Dr. Arturo Beltrán Ortega”, Acapulco, Guerrero, México;Laboratorio de Biomedicina Molecular, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, México;Laboratorio de Citopatología e Histoquímica, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, México;Laboratorio de Citopatología e Histoquímica, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, México;Laboratorio de Investigación en Citopatología e Histoquímica, Unidad Académica de Ciencias Químico Biológicas Universidad Autónoma de Guerrero Avenida Lázaro Cárdenas, Ciudad Universitaria, C.P. 39090, Chilpancingo, Guerrero, México;

关键词: Cervical cancer;    Human Papilloma Virus 16 (HPV-16);    Proteomics;    2D DIGE;    Mass spectrometry;   

DOI  :  10.1186/s12953-016-0099-4

 received in 2015-12-19, accepted in 2016-08-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCervical cancer (CC) is the fourth most common cancer in women worldwide with an estimated 528,000 new cases in 2012. The same year México had an incidence of 13,960 and a mortality of 4769 cases. There are several diagnosis methods of CC; among the most frequents are the conventional Pap cytology (Pap), colposcopy, and visual inspection with acetic acid (VIA), histopathological examination, tests of imaging and detection of high-risk papilloma virus (HR-HPV) with molecular tests (PCR, hybridization, sequencing). Proteomics is a tool for the detection of new biomarkers that can be associated with clinical stage, histological type, prognosis, and/or response to treatment. In this study we performed a comparative analysis of CC cells with normal cervical cells. The proteomic analysis was carried out with the fluorescent two-dimensional electrophoresis (2D-DIGE) technique to subsequently identify differential protein profiles using Decyder Software, and the selected proteins were identified by Mass Spectrometry (MALDI-TOF).ResultsThe proteins that showed an increased expression in cervical cancer in comparison with normal cervix cells were: Mimecan, Actin from aortic smooth muscle and Lumican. While Keratin, type II cytoskeletal 5, Peroxiredoxin-1 and 14-3-3 protein sigma showed a decrease in their protein expression level in cervical cancer in comparison with normal cervix cells.ConclusionsThus, this study was successful in identifying biomarker signatures for cervical cancer, and might provide new insights into the mechanism of CC progression.

【 授权许可】

CC BY   
© The Author(s). 2016