Recombinant E. coli expressing Vitreoscilla haemoglobin prefers aerobic metabolism under microaerobic conditions: A proteome-level study

Journal of biosciences

Kuppamuthu Dharmalingam3¹  Kanak Lata Dikshit²  Bini Ramachandran³ 

[1] National Facility for Clinical Proteomics, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021$$;Institute of Microbial Technology, Sector 39A, Chandigarh 160036$$;Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021$$

关键词: Biological variation analysis;    2D DIGE;    DeCyderTM;    E. coli proteomics;    microaerobic growth;    Vitreoscilla haemoglobin;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Vitreoscilla haemoglobin (VHb) expression in heterologous host was shown to enhance growth and oxygen utilization capabilities under oxygen-limited conditions. The exact mechanism by which VHb enhances the oxygen utilization under oxygen-limiting conditions is still unknown. In order to understand the role of VHb in promoting oxygen utilization, changes in the total protein profile of E. coli expressing the vgb gene under its native promoter was analysed. Two-dimensional difference gel electrophoresis (2D DIGE) was employed to quantify the differentially expressed proteins under oxygen-limiting conditions. Overexpression of proteins involved in aerobic metabolic pathways and suppression of proteins involved in non-oxidative metabolic pathways shown in this study indicates that the cells expressing VHb prefer aerobic metabolic pathways even under oxygen limitation. Under these conditions, the expression levels of proteins involved in central metabolic pathways, cellular adaptation and cell division were also found to be altered. These results imply that Vitreoscilla haemoglobin expression alters aerobic metabolism specifically, in addition to altering proteins involved in other pathways, the significance of which is not clear as of now.

【 授权许可】

Unknown