Impact of three commercial feed formulations on farmed gilthead sea bream (Sparus aurata, L.) metabolism as inferred from liver and blood serum proteomics

Proteome Science

Research Article

Maria Filippa Addis¹  Roberto Anedda¹  Simona Spada¹  Stefania Ghisaura¹  Daniela Pagnozzi¹  Roberto Cappuccinelli¹  Elia Bonaglini¹  Sergio Uzzau¹  Tonina Roggio¹  Grazia Biosa¹ 

[1] Porto Conte Ricerche, S.P. 55 Porto Conte/Capo Caccia Km 8.400, 07041, Tramariglio, Alghero, Italy;

关键词: Gilthead sea bream;    Aquaculture;    Fish feed;    Farmed fish;    Liver proteins;    Serum proteins;    2D DIGE;    Mass spectrometry;    Ingenuity pathway analysis;    Proteomics;   

DOI  :  10.1186/s12953-014-0044-3

 received in 2014-04-17, accepted in 2014-07-31,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe zootechnical performance of three different commercial feeds and their impact on liver and serum proteins of gilthead sea bream (Sparus aurata, L.) were assessed in a 12 week feeding trial. The three feeds, named A, B, and C, were subjected to lipid and protein characterization by gas chromatography (GC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively.ResultsFeed B was higher in fish-derived lipids and proteins, while feeds C and A were higher in vegetable components, although the largest proportion of feed C proteins was represented by pig hemoglobin. According to biometric measurements, the feeds had significantly different impacts on fish growth, producing a higher average weight gain and a lower liver somatic index in feed B over feeds A and C, respectively. 2D DIGE/MS analysis of liver tissue and Ingenuity pathways analysis (IPA) highlighted differential changes in proteins involved in key metabolic pathways of liver, spanning carbohydrate, lipid, protein, and oxidative metabolism. In addition, serum proteomics revealed interesting changes in apolipoproteins, transferrin, warm temperature acclimation-related 65 kDa protein (Wap65), fibrinogen, F-type lectin, and alpha-1-antitrypsin.ConclusionsThis study highlights the contribution of proteomics for understanding and improving the metabolic compatibility of feeds for marine aquaculture, and opens new perspectives for its monitoring with serological tests.

【 授权许可】

Unknown   
© Ghisaura et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.