Antimicrobial resistance profiles of salmonella spp. and escherichia coli isolated from fresh nile tilapia (oreochromis niloticus) fish marketed for human consumption

BMC Microbiology

Research

Johnson K. Kinyua¹  Evans N. Nyaboga²  Edward K. Muge²  Millicent T. Mumbo³  Scholastica G. K. Mathenge⁴  Joshua M. Njiru⁵  Geoffrey Muriira⁵  Bernard Njiraini⁵  Henry Rotich⁵ 

[1] Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Nairobi, Kenya;Department of Biochemistry, University of Nairobi, Nairobi, Kenya;Department of Biochemistry, University of Nairobi, Nairobi, Kenya;Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Nairobi, Kenya;Department of Medical Laboratory Science, Kenyatta University, Nairobi, Kenya;Department of Medical Laboratory Science, Kenyatta University, Nairobi, Kenya;Research and development Department, Kenya Bureau of Standards, Nairobi, Kenya;

关键词: Antimicrobial resistance;    Escherichia coli;    Health risk;    Multi-drug resistant;    Oreochromis niloticus;    S. typhimurium;   

DOI  :  10.1186/s12866-023-03049-8

 received in 2023-01-28, accepted in 2023-10-08,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundSalmonella spp. and pathogenic strains of Escherichia coli are among the major foodborne zoonotic pathogens. These bacterial pathogens cause human illnesses characterized by hemorrhagic colitis, vomiting, nausea, and other agent-related symptoms. The increasing occurrence of antimicrobial resistance in these pathogens is also a serious public health concern globally. Regular surveillance of phenotypes and genotypes of Salmonella spp. and Escherichia coli from animal-derived foods is necessary for effective reduction and control of these foodborne pathogens. This study was conducted to assess the occurrence, antimicrobial resistance, virulence genes and genetic diversity of Salmonella spp. and E. coli isolates from fresh Nile tilapia obtained from retail markets in Nairobi, Kenya.MethodsA total of 68 fresh Nile tilapia fish samples were collected from retail markets and used for isolation of Salmonella spp. and E. coli. Antimicrobial susceptibilities of the isolates weretested by Kirby-Bauer agar disc diffusion method. According to the antimicrobial resistance profiles, the multi-drug resistant isolates were identified by 16 S rRNA sequencing and phylogenetic analysis using the Bayesian inference method. The MDR Salmonella spp. and E. coli isolates were subjected to PCR-based screening for the detection virulence and antibiotic resistance genes.ResultsThe prevalence of contamination of the fish samples with Salmonella spp. and E.coli was 26.47% and 35.29% respectively. Overall phenotypic resistance among the Salmonella spp. ranged from 5.5% for ceftazidime, chloramphenicol, meropenem, nitrofurantoin and streptomycin and 22.2% for penicillin-G. For E. coli phenotypic resistance ranged from 4.2% for ceftazidime and chloramphenicol and 25% for rifampicin. Multi-drug resistance was observed in three Salmonella spp. and two E. coli isolates. Results of 16 S rRNA sequences, sequence alignment and phylogenic trees confirmed the identified MDR isolates as S. typhymurium WES-09, S. typhymurium MAK-22, S. typhimurium EMB-32 and E. coli MAK-26 and E. coli LAN-35. The presence of antibiotic-resistance genes belonging to β-lactamases, tetracycline, sulfonamide, trimethoprim and aminoglycosides-resistant genes were detected in all the identified MDR isolates.ConclusionsThe findings from this study indicate that Nile tilapia (Oreochromis niloticus) sold in retail markets can acts as reservoirs of Salmonella spp. and E. coli pathogens linked to human disease, some of which were multidrug resistance to critically important antimicrobials. Both microorganisms are of zoonotic significance and represent a significant public health risk to the society.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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