Whole-genome analysis of probiotic product isolates reveals the presence of genes related to antimicrobial resistance, virulence factors, and toxic metabolites, posing potential health risks

BMC Genomics

Hong Li¹  Shuyan Liu²  Chao Liu³  Hailong Xiao⁴  Qian Liang⁵  Ya Shi⁵  Huan Chen⁵  Ying Wang⁵  Chengzhi Liu⁵  Xiaoling Zheng⁶  Hong Shen⁶  Jin Wei⁷  Bian Lu⁸  Sebastian Leptihn⁹ 

[1] China National Accreditation Institute for Conformity Assessment, 100062, Beijing, China;Dalian Customs District, 116001, Dalian, Liaoning, China;Department of Orthopaedics, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, 310009, Hangzhou, Zhejiang, China;Hangzhou Institute for Food and Drug Control, 310018, Hangzhou, Zhejiang, China;Key laboratory of Microbial technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, 310012, Hangzhou, Zhejiang, China;NMPA Key laboratory for Testing and Risk Warning of Pharmaceutical Microbiology, Biological Inspection Department, Zhejiang Institute for Food and Drug Control, 310052, Hangzhou, Zhejiang, China;Nordkapp Medical Group, 311121, Hangzhou, Zhejiang, China;Xiaoshan Center for Disease Control and Prevention, 311201, Hangzhou, Zhejiang, China;Zhejiang University-University of Edinburgh Institute, School of Medicine, Zhejiang University, 310058, Hangzhou, Zhejiang, China;

关键词: Probiotic;    Health risk;    Whole genome analysis;    Antimicrobial resistance;    Instability;   

DOI  :  10.1186/s12864-021-07539-9

来源: Springer

【 摘 要 】

BackgroundSafety issues of probiotic products have been reported frequently in recent years. Ten bacterial strains isolated from seven commercial probiotic products on market were evaluated for their safety, by whole-genome analysis.ResultsWe found that the bacterial species of three probiotic products were incorrectly labeled. Furthermore, six probiotic product isolates (PPS) contained genes for the production of toxic metabolites, while another three strains contained virulence genes, which might pose a potential health risk. In addition, three of them have drug-resistance genes, among which two strains potentially displayed multidrug resistance. One isolate has in silico predicted transferable genes responsible for toxic metabolite production, and they could potentially transfer to human gut microflora or environmental bacteria. Isolates of Lactobacillus rhamnosus and Bifidobacterium animalis subsp. lactis are associated with low risk for human consumption. Based on a comparative genome analysis, we found that the isolated Enterococcus faecium TK-P5D clustered with a well-defined probiotic strain, while E. faecalis TK-P4B clustered with a pathogenic strain.ConclusionsOur work clearly illustrates that whole-genome analysis is a useful method to evaluate the quality and safety of probiotic products. Regulatory quality control and stringent regulations on probiotic products are needed to ensure safe consumption and protect human health.

【 授权许可】

CC BY