Whole gene analysis of a genotype G29P[6] human rotavirus strain identified in Central African Republic

BMC Research Notes

Virginie Banga-Mingo¹  Ionela Gouandjika-Vasilache¹  Naga S. Betrapally²  Rashi Gautam²  Mathew D. Esona²  Eric Katz²  Diane Waku-Kouomou²  Michael D. Bowen²  Jose Jaimes² 

[1] Laboratoire Des Virus Entériques/Rougeole, Institut Pasteur de Bangui, BP 923, Ave de L’Indépendance, Bangui, Central African Republic;Viral Gastroenteritis Branch, Division of Viral Diseases, NCIRD, CDC, 1600 Clifton Road, NE, 30329, Atlanta, GA, USA;

关键词: RVA;    Whole genome analysis;    Central African Republic;   

DOI  :  10.1186/s13104-021-05634-4

来源: Springer

【 摘 要 】

ObjectiveRotavirus A (RVA) remains the main causative agent of gastroenteritis in young children and the young of many mammalian and avian species. In this study we describe a RVA strain detected from a 6-month-old child from Central African Republic (CAR).ResultsWe report the 11 open reading frame sequences of a G29-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2 rotavirus strain, RVA/Human-wt/CAR/CAR91/2014/G29P[6]. Nine genes (VP1–VP3, VP6, NSP1–NSP5) shared 90–100% sequence similarities with genogroup 2 rotaviruses. Phylogenetically, backbone genes, except for VP3 and NSP4 genes, were linked with cognate gene sequences of human DS-1-like genogroup 2, hence their genetic origin. The VP3 and NSP4 genes, clustered genetically with both human and animal strains, an indication genetic reassortment human and animal RVA strains has taken place. The VP7 gene shared nucleotide (93–94%) and amino acid (95.5–96.7%) identities with Kenyan and Belgian human G29 strains, as well as to buffalo G29 strain from South Africa, while the VP4 gene most closely resembled P[6]-lineage I strains from Africa and Bangladesh (97%).

【 授权许可】

CC BY