Influence of ejaculation frequency on seminal parameters

Reproductive Biology and Endocrinology

Research

Ashok Agarwal¹  Stefan S. du Plessis²  Mauricio Camargo³  Ángela P. Cadavid⁴  Walter D. Cardona Maya⁴  B. Jose Manuel Mayorga-Torres⁵ 

[1] Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA;Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA;Division of Medical Physiology, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa;Grupo Genética, Regeneración y Cáncer, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Medellín, Antioquia, Colombia;Grupo Reproducción, Departamento de Microbiología y Parasitología, Facultad de Medicina, Sede de Investigación Universitaria, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia;Grupo Reproducción, Departamento de Microbiología y Parasitología, Facultad de Medicina, Sede de Investigación Universitaria, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia;Grupo Genética, Regeneración y Cáncer, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Medellín, Antioquia, Colombia;

关键词: Sperm quality;    Ejaculation frequency;    Seminal parameters;    Reactive oxygen species;    DNA fragmentation;    Mitochondrial membrane potential;    Flow cytometry analysis;   

DOI  :  10.1186/s12958-015-0045-9

 received in 2015-01-16, accepted in 2015-05-13,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundSeveral factors have been shown to influence semen parameters, one of which is sexual abstinence; a clinical criteria included in the semen evaluation to provide maximum sperm quality. The aim of the present study was to assess the effect of a daily ejaculation frequency on conventional and functional semen parameters.MethodsSemen samples were collected daily over a period of two weeks of which every second sample per person was processed and analyzed according to the World Health Organization guidelines. Furthermore, mitochondrial function, intracellular reactive oxygen species production and sperm DNA fragmentation were evaluated by flow cytometry.ResultsTotal sperm count and seminal volume per ejaculation declined and remained decreased for the duration of the daily ejaculation period. However, conventional parameters such as sperm concentration, motility, progressive motility, morphology, vitality and functional parameters such as sperm plasma membrane integrity, mitochondrial membrane potential and DNA fragmentation was not significantly affected and remained similar to the initial measurement throughout the daily ejaculation period. Despite intra- and inter individual variations, the average values of the basic semen parameters remained above the WHO (2010) reference values throughout the daily ejaculation period. Interestingly, a decreasing trend in intracellular ROS production was observed, although statistically not significant.ConclusionsThe study shows that an extended 2 week period of daily ejaculation does not have major clinical effects on conventional and functional seminal parameters.

【 授权许可】

Unknown   
© Mayorga-Torres et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.