Influence of ejaculation frequency on seminal parameters

Reproductive Biology and Endocrinology

Walter D. Cardona Maya⁴  Ángela P. Cadavid⁴  Stefan S. du Plessis²  Ashok Agarwal¹  Mauricio Camargo³  B. Jose Manuel Mayorga-Torres³ 

[1] Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA;Division of Medical Physiology, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa;Grupo Genética, Regeneración y Cáncer, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Medellín, Antioquia, Colombia;Grupo Reproducción, Departamento de Microbiología y Parasitología, Facultad de Medicina, Sede de Investigación Universitaria, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia

关键词: Flow cytometry analysis;    Mitochondrial membrane potential;    DNA fragmentation;    Reactive oxygen species;    Seminal parameters;    Ejaculation frequency;    Sperm quality;   

Others  :  1216412
DOI  :  10.1186/s12958-015-0045-9

 received in 2015-01-16, accepted in 2015-05-13,  发布年份 2015

【 摘 要 】

Background

Several factors have been shown to influence semen parameters, one of which is sexual abstinence; a clinical criteria included in the semen evaluation to provide maximum sperm quality. The aim of the present study was to assess the effect of a daily ejaculation frequency on conventional and functional semen parameters.

Methods

Semen samples were collected daily over a period of two weeks of which every second sample per person was processed and analyzed according to the World Health Organization guidelines. Furthermore, mitochondrial function, intracellular reactive oxygen species production and sperm DNA fragmentation were evaluated by flow cytometry.

Results

Total sperm count and seminal volume per ejaculation declined and remained decreased for the duration of the daily ejaculation period. However, conventional parameters such as sperm concentration, motility, progressive motility, morphology, vitality and functional parameters such as sperm plasma membrane integrity, mitochondrial membrane potential and DNA fragmentation was not significantly affected and remained similar to the initial measurement throughout the daily ejaculation period. Despite intra- and inter individual variations, the average values of the basic semen parameters remained above the WHO (2010) reference values throughout the daily ejaculation period. Interestingly, a decreasing trend in intracellular ROS production was observed, although statistically not significant.

Conclusions

The study shows that an extended 2 week period of daily ejaculation does not have major clinical effects on conventional and functional seminal parameters.

【 授权许可】

   
2015 Mayorga-Torres et al.; licensee BioMed Central.

【 图 表 】

Fig. 1.

Fig. 2.

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