Molecules produced by probiotics prevent enteric colibacillosis in pigs

BMC Veterinary Research

Research Article

Chuan Wang¹  Rocio Morales¹  Sapana Sharma¹  Mansel William Griffiths¹  Akalate Tessema¹  Zlatko Kovač²  Ricardo Nordeste³ 

[1] Canadian Research Institute for Food Safety, University of Guelph, N1G 2W1, Guelph, ON, Canada;Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870, Dyrlægevej 16, Copenhagen, Frederiksberg C, Denmark;MicroSintesis Inc., Duffy Research Centre, NRC Building 28, 550 University Ave, Charlottetown, PE, Canada;

关键词: E. coli;    Probiotics;    Bioactive molecules;    Proteobiotics;    Pigs;    Enteric colibacillosis;    Anti-virulence;   

DOI  :  10.1186/s12917-017-1246-6

 received in 2017-03-22, accepted in 2017-10-30,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundWith the advent of antimicrobial resistance in animal pathogens, novel methods to combat infectious diseases are being sought. Among these, probiotics have been proposed as a means of promoting animal health but problems with their use has been reported. Research has demonstrated that bioactive molecules produced during the growth of certain probiotics interfere with bacterial cell-to-cell communication, which consequently results in an attenuation of virulence in a number of pathogens, including E. coli. The objective of this study was to determine the efficacy of the bioactive molecules, termed proteobiotics, produced by Lactobacillus acidophilus in preventing enterotoxigenic E, coli (ETEC) infection in pigs, which is the etiological agent for enteric colibacillosis, a common disease of nursing and young pigs.ResultsTo achieve this, piglets were fed a preparation of the bioactive at four levels: 0, 0.5×, 1.0× and 2.0× for 7 days prior to challenge with E. coli K88. There were 36 pigs (18 gilts and 18 barrows) per treatment, resulting in 144 piglets in total for the study. Each pen had 6 piglets (3 gilts and 3 barrows). Only piglets with no physical abnormality or conditions were used in the trial and intact male piglets and ridglings were excluded. The bioactive continued to be fed to the pigs post-challenge. Based of fecal and demeanour scores, pigs fed the low and high dose of the proteobiotic were significanlty less likely to show symptoms of illness than pigs fed no bioactive. While not being significant, the weight gain of pigs given the proteobiotics was improved. At day 4 following challenge, almost 50% of piglets that did not receive the proteobiotic were shedding ETEC in their feces, compared with about 15% of animals receiving the supplement. There was also an indication that the proteobiotics reduced colonization of the ileum by E. coli K88 and improved gut health.ConclusionThis study indicates that the bioactive molecules produced by L. acidophilus reduces incidence of enteric colibacillosis in pigs and their use on farms would help to reduce antibiotic use.

【 授权许可】

CC BY   
© The Author(s). 2017