Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats

Journal of Biomedical Science

Research

Jia-Fwu Shyu¹  Ling-Chen Tai²  Yi-Ming Shyr²  Zen-Chung Weng³  Hwai-Shi Wang⁴  Tien-Hua Chen⁵  Pei-Jiun Tsai⁶ 

[1] Department of Biology and Anatomy, National Defense Medical Center, 161 Ming Chuan E. Road Section 6, 114, Taipei, Republic of China;Department of Surgery, Veteran General Hospital, Taipei, Republic of China;Division of Cardiovascular Surgery, Cardiovascular Center, Taipei Medical University Hospital, Taipei, Republic of China;Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming University, 201 Shih-Pai Road Section 2, 112, Taipei, Republic of China;Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming University, 201 Shih-Pai Road Section 2, 112, Taipei, Republic of China;Department of Surgery, Veteran General Hospital, Taipei, Republic of China;Institute of Clinical Medicine, National Yang-Ming University, Taipei, Republic of China;Department of Emergency, Division of Surgery, Veteran General Hospital, Taipei, Republic of China;

关键词: Mesenchymal stem cell;    Portal vein;    Insulin-producing cells;    Transplant;   

DOI  :  10.1186/1423-0127-19-47

 received in 2012-02-11, accepted in 2012-04-30,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAlthough diabetes mellitus (DM) can be treated with islet transplantation, a scarcity of donors limits the utility of this technique. This study investigated whether human mesenchymal stem cells (MSCs) from umbilical cord could be induced efficiently to differentiate into insulin-producing cells. Secondly, we evaluated the effect of portal vein transplantation of these differentiated cells in the treatment of streptozotocin-induced diabetes in rats.MethodsMSCs from human umbilical cord were induced in three stages to differentiate into insulin-producing cells and evaluated by immunocytochemistry, reverse transcriptase, and real-time PCR, and ELISA. Differentiated cells were transplanted into the liver of diabetic rats using a Port-A catheter via the portal vein. Blood glucose levels were monitored weekly.ResultsHuman nuclei and C-peptide were detected in the rat liver by immunohistochemistry. Pancreatic β-cell development-related genes were expressed in the differentiated cells. C-peptide release was increased after glucose challenge in vitro. Furthermore, after transplantation of differentiated cells into the diabetic rats, blood sugar level decreased. Insulin-producing cells containing human C-peptide and human nuclei were located in the liver.ConclusionThus, a Port-A catheter can be used to transplant differentiated insulin-producing cells from human MSCs into the portal vein to alleviate hyperglycemia among diabetic rats.

【 授权许可】

Unknown   
© Tsai et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.