Journal of Biomedical Science | |
Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats | |
Tien-Hua Chen5  Jia-Fwu Shyu3  Ling-Chen Tai5  Zen-Chung Weng4  Yi-Ming Shyr5  Hwai-Shi Wang1  Pei-Jiun Tsai2  | |
[1] Institute of Anatomy and Cell Biology, School of Medicine, National Yang Ming University, 201 Shih-Pai Road Section 2, Taipei, 112, Republic of China;Department of Emergency, Division of Surgery, Veteran General Hospital, Taipei, Republic of China;Department of Biology and Anatomy, National Defense Medical Center, 161 Ming Chuan E. Road Section 6, Taipei, 114, Republic of China;Division of Cardiovascular Surgery, Cardiovascular Center, Taipei Medical University Hospital, Taipei, Republic of China;Department of Surgery, Veteran General Hospital, Taipei, Republic of China | |
关键词: Transplant; Insulin-producing cells; Portal vein; Mesenchymal stem cell; | |
Others : 825116 DOI : 10.1186/1423-0127-19-47 |
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received in 2012-02-11, accepted in 2012-04-30, 发布年份 2012 | |
【 摘 要 】
Background
Although diabetes mellitus (DM) can be treated with islet transplantation, a scarcity of donors limits the utility of this technique. This study investigated whether human mesenchymal stem cells (MSCs) from umbilical cord could be induced efficiently to differentiate into insulin-producing cells. Secondly, we evaluated the effect of portal vein transplantation of these differentiated cells in the treatment of streptozotocin-induced diabetes in rats.
Methods
MSCs from human umbilical cord were induced in three stages to differentiate into insulin-producing cells and evaluated by immunocytochemistry, reverse transcriptase, and real-time PCR, and ELISA. Differentiated cells were transplanted into the liver of diabetic rats using a Port-A catheter via the portal vein. Blood glucose levels were monitored weekly.
Results
Human nuclei and C-peptide were detected in the rat liver by immunohistochemistry. Pancreatic β-cell development-related genes were expressed in the differentiated cells. C-peptide release was increased after glucose challenge in vitro. Furthermore, after transplantation of differentiated cells into the diabetic rats, blood sugar level decreased. Insulin-producing cells containing human C-peptide and human nuclei were located in the liver.
Conclusion
Thus, a Port-A catheter can be used to transplant differentiated insulin-producing cells from human MSCs into the portal vein to alleviate hyperglycemia among diabetic rats.
【 授权许可】
2012 Tsai et al.; licensee BioMed Central Ltd.
【 预 览 】
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20140713054646116.pdf | 1926KB | download | |
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Figure 5. | 11KB | Image | download |
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Figure 3. | 33KB | Image | download |
Figure 2. | 16KB | Image | download |
Figure 1. | 39KB | Image | download |
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