Studying the influence of nanodiamonds over the elasticity of polymer/nanodiamond composites for biomedical application

18th International School on Condensed Matter Physics: "Challenges of Nanoscale Science: Theory, Materials, Applications"

Hikov, T.^1 ; Mitev, D.^1 ; Radeva, E.^1 ; Iglic, A.^2 ; Presker, R.^2 ; Daniel, M.^3 ; Sepitka, J.^3 ; Krasteva, N.^4 ; Keremidarska, M.^4 ; Cvetanov, I.^1 ; Pramatarova, L.^1

Georgi Nadjakov Institute of Solid State Physics, BAS, Sofia

1784, Bulgaria^1

University of Ljubljana, Ljubljana

1000, Slovenia^2

Faculty of Mechanical Engineering, Czech Technical University, Prague

166 07, Czech Republic^3

Institute of Biophysics and Biomedical Engineering, BAS, Sofia

1113, Bulgaria^4

关键词: Biological components;    Biomedical applications;    Detonation nanodiamond;    Focus of researches;    Hexamethyldisiloxane;    Mechanically stable;    Mesenchymal stem cell;    Spatial organization;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/558/1/012060/pdf
DOI  :  10.1088/1742-6596/558/1/012060

来源: IOP

【 摘 要 】

The combined unique properties offered by organic and inorganic constituents within a single material on a nanoscale level make nanocomposites attractive for the next generation of biocompatible materials. The composite materials of the detonation nanodiamond/polymer type possess spatial organization of components with new structural features and physical properties, as well as complex functions due to the strong synergistic effects between the nanoparticles and the polymer [1]. The plasma polymerization (PP) method was chosen to obtain composites of silicon-based polymers, in which detonation generated nanodiamond (DND) particles were incorporated. The composite layers are homogeneous, chemically resistant, thermally and mechanically stable, thus allowing a large amount of biological components to be loaded onto their surface and to be used in tissue engineering, regenerative medicine, implants, stents, biosensors and other medical and biological devices. Mesenchymal stem cells (MSCs) are the main focus of research in regenerative medicine due to their extraordinary potential to differentiate into different kinds of cells including osteoblasts, which are needed for various bone disease treatments. However, for optimal usage of MSCs knowledge about the factors that influence their initial distribution in the human system, tissue-specific activation and afterwards differentiation into osteoblasts is required. In recent studies it was found that one of these factors is the elasticity of the substrates [2]. The choice of the proper material which specifically guides the differentiation of stem cells even in the absence of growth factors is very important when building modern strategy for bone regeneration. One of the reasons for there not being many studies in this area worldwide is the lack of suitable biomaterials which support these kinds of experiments. The goal of this study is to create substrates suitable for cell culture with a range of mechanical properties (namely elasticity and hardness) using composite layers (PPHMDS-DND) of plasma polymerized (PP) hexamethyldisiloxane (HMDS) and detonation generated nanodiamond (DND). The samples' elastic modulae and hardness were measured by CSM Ultra Nanoindentation Tester.