Epidemiology of Plasmodium infections in Flores Island, Indonesia using real-time PCR

Malaria Journal

Research

Linda J Wammes¹  Maria Yazdanbakhsh¹  Lisette van Lieshout¹  Eric AT Brienen¹  Erliyani Sartono¹  Jaco J Verweij²  Taniawati Supali³  Firdaus Hamid⁴  Aprilianto E Wiria⁵  Maria MM Kaisar⁵  Adrian JF Luty⁶ 

[1] Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands;Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands;Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands;Department of Parasitology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia;Department of Parasitology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia;Department of Microbiology, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia;Department of Parasitology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia;Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands;Unités Mixtes de Recherche, Institut de Recherche pour le Développement, Mère et Enfant Face aux Infections, Tropicales, France;

关键词: Malaria;    Plasmodium falciparum;    Plasmodium vivax;    Plasmodium malariae;    Real-time PCR;    Diagnosis;    Flores;   

DOI  :  10.1186/1475-2875-12-169

 received in 2013-01-29, accepted in 2013-05-16,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundDNA-based diagnostic methods have been shown to be highly sensitive and specific for the detection of malaria. An 18S-rRNA-based, real-time polymerase chain reaction (PCR) was used to determine the prevalence and intensity of Plasmodium infections on Flores Island, Indonesia.MethodsMicroscopy and real-time multiplex PCR for the detection of Plasmodium species was performed on blood samples collected in a population-based study in Nangapanda Flores Island, Indonesia.ResultsA total 1,509 blood samples were analysed. Real-time PCR revealed prevalence for Plasmodium falciparum, Plasmodium vivax, and Plasmodium malariae to be 14.5%, 13.2%, and 1.9% respectively. Sub-microscopic parasitaemia were found in more than 80% of all positive cases. The prevalence of P. falciparum and P. vivax was significantly higher in subjects younger than 20 years (p ≤ 0.01). In the present study, among non-symptomatic healthy individuals, anaemia was strongly correlated with the prevalence and load of P. falciparum infections (p ≤ 0.01; p = 0.02) and with the load of P. vivax infections (p = 0.01) as detected with real-time PCR. Subjects with AB blood group tend to have a higher risk of being infected with P. falciparum and P. vivax when compared to other blood groups.ConclusionThe present study has shown that real-time PCR provides more insight in the epidemiology of Plasmodium infections and can be used as a monitoring tool in the battle against malaria. The unsurpassed sensitivity of real-time PCR reveals that sub microscopic infections are common in this area, which are likely to play an important role in transmission and control.Trial registrationTrials number http://www.controlled-trials.com/ISRCTN83830814

【 授权许可】

Unknown   
© Kaisar et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.