Complexity of malaria transmission dynamics in the Brazilian Atlantic Forest

Current Research in Parasitology and Vector-Borne Diseases

卷:1

Fabiana Santos Silva¹  Walter Ceretti-Junior²  Licia Natal Fernandes²  Gabriel Zorello Laporta³  Margoth Ramos Garnica⁴  Eduardo Evangelista⁵  Marcello Schiavo Nardi⁵  Juliana Laurito Summa⁵  Mauro Toledo Marrelli⁵  Renildo Teixeira⁵  Ana Carolina Loss⁶  Julyana Cerqueira Buery⁶  M. Andreína Pacheco⁷  Crispim Cerutti Jr.⁸  Luis Filipe Mucci⁹  Igor Lucoves Sicchi⁹  Antônio Ralph Medeiros-Sousa⁹  Ana Maria Ribeiro de Castro Duarte⁹  Maria Anice Mureb Sallum¹⁰  Ananias A. Escalante¹⁰  Izilda Curado¹¹  Aristides Fernandes¹¹ 

[1] Corresponding author. Laboratório de Protozoologia, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, SP, Brazil.;

[2] Superintendência de Controle de Endemias, Secretaria de Estado da Saúde, SP, Brazil;

[3] Biology Department/Institute of Genomics and Evolutionary Medicine (iGEM), Temple University, Philadelphia, PA, USA;

[4] Centro de Controle de Zoonoses, Secretaria Municipal da Saúde, São Paulo, SP, Brazil;

[5] Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brazil;

[6] Divisão da Fauna Silvestre, Secretaria do Verde e Meio Ambiente da Prefeitura de São Paulo, São Paulo, SP, Brazil;

[7] Instituto Nacional da Mata Atlântica, Santa Teresa, ES, Brazil;

[8] Laboratório de Nefrologia Celular, Genética e Molecular (LIM/29), Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil;

[9] Laboratório de Protozoologia, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, SP, Brazil;

[10] Programa de Pós-Graduação em Doenças Infecciosas, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brazil;

关键词: Anopheles;    Malaria;    Plasmodium;    Plasmodium falciparum;    Plasmodium malariae;    Plasmodium vivax;   

DOI  :  

来源: DOAJ

【 摘 要 】

Plasmodium malariae and Plasmodium vivax are protozoan parasites that can cause malaria in humans. They are genetically indistinguishable from, respectively, Plasmodium brasilianum and Plasmodium simium, i.e. parasites infecting New World non-human primates in South America. In the tropical rainforests of the Brazilian Atlantic coast, it has long been hypothesized that P. brasilianum and P. simium in platyrrhine primates originated from P. malariae and P. vivax in humans. A recent hypothesis proposed the inclusion of Plasmodium falciparum into the transmission dynamics between humans and non-human primates in the Brazilian Atlantic tropical rainforest. Herein, we assess the occurrence of human malaria in simians and sylvatic anophelines using field-collected samples in the Capivari-Monos Environmental Protection Area from 2015 to 2017. We first tested simian blood and anopheline samples. Two simian (Aloutta) blood samples (18%, n = 11) showed Plasmodium cytb DNA sequences, one for P. vivax and another for P. malariae. From a total of 9,416 anopheline females, we found 17 pools positive for Plasmodium species with a 18S qPCR assay. Only three showed P. cytb DNA sequence, one for P. vivax and the others for rodent malaria species (similar to Plasmodium chabaudi and Plasmodium berghei). Based on these results, we tested 25 rodent liver samples for the presence of Plasmodium and obtained P. falciparum cytb DNA sequence in a rodent (Oligoryzomys sp.) liver. The findings of this study indicate complex malaria transmission dynamics composed by parallel spillover-spillback of human malaria parasites, i.e. P. malariae, P. vivax, and P. falciparum, in the Brazilian Atlantic forest.

【 授权许可】

Unknown