Genotypic characterization of drug resistant Mycobacterium tuberculosis in Quebec, 2002-2012

BMC Microbiology

Research Article

Élyse Boivin¹  Hafid Soualhine¹  Émilie Bélanger-Trudelle¹  Huguette Fauchon¹  Joanna Spinato²  Cécile Tremblay³ 

[1] Laboratoire de santé publique du Quebec, 20045 chemin Sainte-Marie, H9X 3R5, Sainte-Anne de Bellevue, Quebec, Canada;McGill University, Montreal, Quebec, Canada;Present address: Public Health Ontario Laboratory, M5G 1 M1, Toronto, Ontario, Canada;Université de Montreal, Montreal, Quebec, Canada;

关键词: M. tuberculosis;    Drug-resistance;    rpoB;    inhA;    katG;    pncA;    embB;   

DOI  :  10.1186/s12866-016-0786-4

 received in 2016-02-01, accepted in 2016-07-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe increasing emergence of drug-resistant tuberculosis presents a threat to the effective control of tuberculosis (TB). Rapid detection of drug-resistance is more important than ever to address this scourge. The purpose of this study was to genotypically characterize the first-line antitubercular drug-resistant isolates collected over 11 years in Quebec.ResultsThe main mutations found in our resistant strains collection (n = 225) include: the S315T substitution in katG (50.2 %), the -15 C/T mutation in the inhA promoter (29 %); the S531L substitution in rpoB (43 %); the deletion 8 bp 446 / + R140S in pncA (72.9 %); the M306I (35.7 %) and M306V (21.4 %) substitutions in embB. Ten of the mutations in katG and 4 mutations identified in pncA were previously undescribed.ConclusionScreening of mutations conferring resistance to first-line antituberculous drugs using DNA-sequencing approach seems to be feasible and would drastically shorten the time to determine the resistance profile compared to the proportion method.

【 授权许可】

CC BY   
© The Author(s). 2016