In vitro and in vivo anti-malarial activity of Boerhavia elegans and Solanum surattense

Malaria Journal

Research

Soroush Sardari¹  Navid Dinparas Djadidt²  Ali Ramazani²  Sedigheh Zakeri²  Nastaran Khodakarim² 

[1] Drug Discovery and Bioinformatics Group, Biotechnology Research Center of Institut Pasteur of Iran, Tehran, Iran;Malaria and Vector Research Group (MVRG), Biotechnology Research Center, Institut Pasteur of Iran, Tehran, Iran, P.O. BOX 1316943551, Pasteur Avenue, Tehran, Iran;

关键词: Malaria;    Brine Shrimp;    Selectivity Index;    Lumefantrine;    Dichloromethane Fraction;   

DOI  :  10.1186/1475-2875-9-124

 received in 2009-12-22, accepted in 2010-05-12,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThere is an urgent need to identify new anti-malarial drug targets for both prophylaxis and chemotherapy, due to the increasing problem of drug resistance to malaria parasites. In the present study, the aim was to discover novel, effective plant-based extracts for the activity against malaria.MethodsTen plants found in Iran were selected by ethnobotanical survey of medicinal plants. The crude ethanolic extracts were tested for in vitro anti-plasmodial activity against two strains of Plasmodium falciparum: K1 (chloroquine-resistant strain) and CY27 (chloroquine-sensitive strain), using the parasite lactate dehydrogenase (pLDH) assay. The anti-plasmodial activity of the extracts was also assessed in the 4-day suppressive anti-malarial assay in mice inoculated with Plasmodium berghei (ANKA strain). Crude ethanolic extracts showed good anti-plasmodial activity were further fractionated by partitioning in water and dichloromethane.ResultsOf 10 plant species assayed, three species: Boerhavia elegans (Choisy), Solanum surattense (Burm.f.) and Prosopis juliflora (Sw.) showed promising anti-plasmodial activity in vitro (IC50 ≤ 50 μg/ml) and in vivo with no toxicity. The dichloromethane fraction of three extracts revealed stronger anti-plasmodial activity than the total extracts.ConclusionAnti-plasmodial activities of extracts of B. elegans and S. surattense are reported for the first time.

【 授权许可】

Unknown   
© Ramazani et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.