Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity

Malaria Journal

Research

Minjie Zhao¹  Saïd Ennahar¹  Eric Marchioni¹  Serigne O Sarr²  Sylvie Perrotey³  Ermanno Candolfi³  Yérim M Diop⁴  Ibrahima Fall⁵ 

[1] Equipe de Chimie Analytique des Molécules BioActives IPHC-LC4, UMR 7178, Faculté de Pharmacie, 74, route du Rhin, 67400, Illkirch, France;Equipe de Chimie Analytique des Molécules BioActives IPHC-LC4, UMR 7178, Faculté de Pharmacie, 74, route du Rhin, 67400, Illkirch, France;Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine et de Pharmacie, Université Cheikh Anta DIOP, B.P 5005, Dakar-Fann, Sénégal;Institut de Parasitologie et de Pathologie Tropicale, Université de Strasbourg, EA 4438, 3 rue Koeberlé, 67000, Strasbourg, France;Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine et de Pharmacie, Université Cheikh Anta DIOP, B.P 5005, Dakar-Fann, Sénégal;Laboratoire de Pharmacognosie et Botanique, Faculté de Médecine et de Pharmacie, Université Cheikh Anta DIOP, B.P 5005, Dakar-Fann, Sénégal;

关键词: Malaria;    Chloroquine;    Stem Bark;    Selectivity Index;    Haemolytic Activity;   

DOI  :  10.1186/1475-2875-10-85

 received in 2010-09-29, accepted in 2011-04-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundWith the aim of discovering new natural active extracts against malaria parasites, Icacina senegalensis was selected after an ethnopharmacological survey conducted on plants used in traditional malaria treatment in Senegal.MethodsDifferent concentrations of the plant extract and fractions were tested on synchronized Plasmodium falciparum cultures at the ring stage using the parasite lactate dehydrogenase assay. Their haemolytic activity and in vitro cytoxicity were evaluated. The chromatographic profiles of active fractions were also established.ResultsThe plant extract and fractions revealed anti-plasmodial activity (IC50 < 5 μg/mL) with no toxicity (Selectivity indexes >10). The dichloromethane fraction showed stronger anti-plasmodial activity than the total extract.ConclusionAnti-plasmodial activity and toxicity of I. senegalensis are reported for the first time and showed promising results in malaria field research.

【 授权许可】

Unknown   
© Sarr et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.