Effect of selected local medicinal plants on the asexual blood stage of chloroquine resistant Plasmodium falciparum

BMC Complementary and Alternative Medicine

Research Article

Rosnani Ali¹  Siti Habsah Shiekh Zahari¹  Noor Rain Abdullah¹  Zakiah Ismail¹  Mohd Ridzuan Mohd Abd Razak¹  Adlin Afzan¹  Nur Fasihah Amir Jalaluddin¹  Mohd Isa Wasiman¹ 

[1] Herbal Medicine Research Centre, Institute for Medical Research, 50588, Jalan PahangKuala Lumpur, Malaysia;

关键词: Malaria;    Antimalarial Drug;    Selectivity Index;    MeOH Extract;    Antiplasmodial Activity;   

DOI  :  10.1186/1472-6882-14-492

 received in 2014-03-03, accepted in 2014-12-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe development of resistant to current antimalarial drugs is a major challenge in achieving malaria elimination status in many countries. Therefore there is a need for new antimalarial drugs. Medicinal plants have always been the major source for the search of new antimalarial drugs. The aim of this study was to screen selected Malaysian medicinal plants for their antiplasmodial properties.MethodsEach part of the plants were processed, defatted by hexane and sequentially extracted with dichloromethane, methanol and water. The antiplasmodial activities of 54 plant extracts from 14 species were determined by Plasmodium falciparum Histidine Rich Protein II ELISA technique. In order to determine the selectivity index (SI), all plant extracts demonstrating a good antiplasmodial activity were tested for their cytotoxicity activity against normal Madin-Darby Bovine Kidney (MDBK) cell lines by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay.ResultsTwenty three extracts derived from Curcuma zedoaria (rhizome), Curcuma aeruginosa (rhizome), Alpinia galanga (rhizome), Morinda elliptica (leaf), Curcuma mangga (rhizome), Elephantopus scaber (leaf), Vitex negundo (leaf), Brucea javanica (leaf, root and seed), Annona muricata (leaf), Cinnamomun iners (leaf) and Vernonia amygdalina (leaf) showed promising antiplasmodial activities against the blood stage chloroquine resistant P. falciparum (EC50 < 10 μg/ml) with negligible toxicity effect to MDBK cells in vitro (SI ≥10).ConclusionThe extracts belonging to eleven plant species were able to perturb the growth of chloroquine resistant P. falciparum effectively. The findings justified the bioassay guided fractionation on these plants for the search of potent antimalarial compounds or formulation of standardized extracts which may enhance the antimalarial effect in vitro and in vivo.

【 授权许可】

CC BY   
© Mohd Abd Razak et al.; licensee BioMed Central. 2014