Anti-inflammatory effects of an injectable copolymer of fatty acids (Ara 3000 beta®) in joint diseases

Journal of Inflammation

Research

Céline Quesnelle¹  Nicolas Girard¹  Karim Boumediene¹  Catherine Baugé¹  Eva Lhuissier¹  Gérard Ewert² 

[1] Normandie Univ, Caen, France;UNICAEN, EA4652 MILPAT UFR de médecine, Université de Caen, Caen cedex 5, CS14032, Caen, France;Sexmoor Laboratoires, 13 120 Saint Remy de Provence, France;

关键词: Cytokines;    Drug therapy;    Extracellular matrix;    Fatty acid;    Gene expression;    Inflammation;    Arthritis;    Osteoarthritis;    MMPs;    Joints;   

DOI  :  10.1186/s12950-015-0062-7

 received in 2014-08-14, accepted in 2015-02-16,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIn inflammatory joint disease, such as osteoarthritis or arthritis, there is an increased level of pro-inflammatory cytokines, such as interleukin-1β. These cytokines stimulate the expression and release of matrix metalloproteases (MMP), leading to the degradation of cartilage extracellular matrix and subsequently mobility difficulty and suffering for patients. The aim of this study was to examine the therapeutic potential of a fatty acid copolymer in in vitro and in vivo models of cartilage inflammation.MethodsInflammation was mimicked in vitro by treatment of human articular chondrocytes with interleukin-1β. Effects of a co-treatment with a copolymer of fatty acids (Ara 3000 beta®) were determined by evaluating MMP production by RT-PCR and ELISA, NO release by Griess assay, and PGE2 expression by ELISA. In addition, in vivo analysis (evolution of weight and edema) were also performed after injection of Freund adjuvant in rats treated or not with the copolymer of fatty acids.ResultsThe copolymer of fatty acids clearly reduces inflammation in joint. In vitro, it impairs IL1 stimulated-MMP production and release, as well as the release of NO and PGE2 and the activation of NFκB. Furthermore, in vivo experiments using adjuvant induced-arthritis corroborates the anti-inflammatory effects of the copolymer of fatty acids, with a reduction of edemas, erythemas and ankylosis in arthritic rats.ConclusionsThe results support the hypothesis that a copolymer of fatty acids, such as Ara 3000 beta®, is a powerful anti-inflammatory compounds, suggesting that it has a potential for preventing cartilage degradation associated with chronic inflammatory joint disease.

【 授权许可】

CC BY   
© Baugé et al.; licensee BioMed Central. 2015