Verification of clinically diagnosed cases during malaria elimination programme in Guizhou Province of China

Malaria Journal

Research

Lidan Lu¹  Yan Geng¹  Yuting Huang¹  Ping He¹  Jianjun Xu¹  He Yan²  Ning Xiao²  Zhigui Xia²  Jianhai Yin²  Shuisen Zhou² 

[1] Guizhou Provincial CDC, 550004, Guiyang, China;National Institute of Parasitic Diseases, Chinese Centre for Disease Control and Prevention (NIPD, China CDC), 200025, Shanghai, China;Key Laboratory of Parasite and Vector Biology, MOH, China, 200025, Shanghai, China;WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, 200025, Shanghai, China;

关键词: Case verification;    Malaria elimination;    UT-PCR;    Nested PCR;    Guizhou Province;   

DOI  :  10.1186/1475-2875-12-130

 received in 2012-12-05, accepted in 2013-04-12,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundChina is implementing a National Malaria Elimination Programme. A high proportion of clinically diagnosed malaria cases is reported in some provinces of China. In order to understand the exact situation and make clear the nature of these patients, it is of much importance to make case verifications, particularly from the pathogenic perspective.MethodsGuizhou Province was targeted because of its high proportion of clinically diagnosed malaria cases. After random selection of around 10% of malaria cases from 1 May 2011 to 30 April 2012, reported through the national web-based case reporting system from this province, field verifications were made on 14–17 May 2012 as follows. Firstly, the reported information of each case was rechecked with the onsite case registrations and investigation forms, and an in-depth interview was conducted with each patient. Secondly, the patient’s blood smears kept by local CDC were cross-checked microscopically by a national experienced microscopist. Thirdly, two kinds of polymerase chain reaction (PCRs). including Tag-primer nested/multiplex PCR (UT-PCR) based on cytochrome oxidase gene (cox I) and nested PCR based on 18s rRNA gene were performed simultaneously using local CDC kept filter paper of dry blood samples to identify the Plasmodium spp.ResultsTwelve out of 152 malaria cases were selected, including nine clinically diagnosed malaria cases, two confirmed falciparum malaria cases and one confirmed vivax malaria case. The original case documents on the site were completely in conformity with their reported data, and all the patients recalled their malaria symptoms and being cured only after consuming the corresponding anti-malarial drugs. Moreover, the re-examination results of microscopy and PCR were exactly in agreement with the original tests.DiscussionNo inconsistent results were found against the reported case information in the present study and the reasons for clinically diagnosed patients remains unclear. Uniform and standardized sample collection and processing should be trained among clinicians, more sensitive and specific techniques should be explored to used in malaria diagnosis. A further study is needed in order to be more observationally focussed rather than retrospective.

【 授权许可】

Unknown   
© Yin et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.