The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro

BMC Musculoskeletal Disorders

Research Article

Jens Schaumburger¹  Joachim Grifka¹  Tobias Renkawitz¹  Philipp Lechler¹  Clemens Baier¹  Susanne Grässel²  Sanjeevi Balakrishnan³  Rainer H Straub⁴ 

[1] Department of Orthopedic Surgery, University of Regensburg, Asklepios Klinikum Bad Abbach, Kaiser Karl V Allee 3, 93077, Bad Abbach, Germany;Department of Orthopedic Surgery, University of Regensburg, Asklepios Klinikum Bad Abbach, Kaiser Karl V Allee 3, 93077, Bad Abbach, Germany;Centre for Biomedical Technology, BioPark 1, Josef-Engert-Str. 9, 93053, Regensburg, Germany;Division of Immunology and Inflammation (Medicine), Imperial College London, Hammersmith Hospital, Du Cane Road, W12 0HS, London, UK;Laboratory of Experimental Rheumatology and Neuroendocrino-Immunology, Division of Rheumatology, Department of Internal Medicine I, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany;

关键词: apoptosis;    chondrocyte;    osteoarthritis;    proliferation;    survivin;   

DOI  :  10.1186/1471-2474-12-150

 received in 2011-04-06, accepted in 2011-07-05,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundRegulation of cell death and cell division are key processes during chondrogenesis and in cartilage homeostasis and pathology. The oncogene survivin is considered to be critical for the coordination of mitosis and maintenance of cell viability during embryonic development and in cancer, and is not detectable in most adult differentiated tissues and cells. We analyzed survivin expression in osteoarthritic cartilage and its function in primary human chondrocytes in vitro.MethodsSurvivin expression was analyzed by immunoblotting and quantitative real-time PCR. The localization was visualized by immunofluorescence. Survivin functions in vitro were investigated by transfection of a specific siRNA.ResultsSurvivin was expressed in human osteoarthritic cartilage, but was not detectable in macroscopically and microscopically unaffected cartilage of osteoarthritic knee joints. In primary human chondrocyte cultures, survivin was localized to heterogeneous subcellular compartments. Suppression of survivin resulted in inhibition of cell cycle progression and sensitization toward apoptotic stimuli in vitro.ConclusionsThe present study indicates a role for survivin in osteoarthritic cartilage and human chondrocytes. In vitro experiments indicated its involvement in cellular division and viability. Learning more about the functions of survivin in chondrocyte biology might further help toward understanding and modulating the complex processes of cartilage pathology and regeneration.

【 授权许可】

CC BY   
© Lechler et al; licensee BioMed Central Ltd. 2011