The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation in vitro

BMC Musculoskeletal Disorders

Tobias Renkawitz⁴  Rainer H Straub¹  Joachim Grifka⁴  Clemens Baier⁴  Susanne Grässel²  Jens Schaumburger⁴  Sanjeevi Balakrishnan³  Philipp Lechler⁴ 

[1] Laboratory of Experimental Rheumatology and Neuroendocrino-Immunology, Division of Rheumatology, Department of Internal Medicine I, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany;Centre for Biomedical Technology, BioPark 1, Josef-Engert-Str. 9 93053 Regensburg, Germany;Division of Immunology and Inflammation (Medicine), Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0HS, UK;Department of Orthopedic Surgery, University of Regensburg, Asklepios Klinikum Bad Abbach, Kaiser Karl V Allee 3, 93077 Bad Abbach, Germany

关键词: survivin;    proliferation;    osteoarthritis;    chondrocyte;    apoptosis;   

Others  :  1152820
DOI  :  10.1186/1471-2474-12-150

 received in 2011-04-06, accepted in 2011-07-05,  发布年份 2011

【 摘 要 】

Background

Regulation of cell death and cell division are key processes during chondrogenesis and in cartilage homeostasis and pathology. The oncogene survivin is considered to be critical for the coordination of mitosis and maintenance of cell viability during embryonic development and in cancer, and is not detectable in most adult differentiated tissues and cells. We analyzed survivin expression in osteoarthritic cartilage and its function in primary human chondrocytes in vitro.

Methods

Survivin expression was analyzed by immunoblotting and quantitative real-time PCR. The localization was visualized by immunofluorescence. Survivin functions in vitro were investigated by transfection of a specific siRNA.

Results

Survivin was expressed in human osteoarthritic cartilage, but was not detectable in macroscopically and microscopically unaffected cartilage of osteoarthritic knee joints. In primary human chondrocyte cultures, survivin was localized to heterogeneous subcellular compartments. Suppression of survivin resulted in inhibition of cell cycle progression and sensitization toward apoptotic stimuli in vitro.

Conclusions

The present study indicates a role for survivin in osteoarthritic cartilage and human chondrocytes. In vitro experiments indicated its involvement in cellular division and viability. Learning more about the functions of survivin in chondrocyte biology might further help toward understanding and modulating the complex processes of cartilage pathology and regeneration.

【 授权许可】

   
2011 Lechler et al; licensee BioMed Central Ltd.

【 图 表 】

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