Effect of recombinant PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix: a pilot study in rat calvarial defects

BMC Oral Health

Research Article

Roberta Okamoto¹  Reinhard Gruber²  Eloá R. Luvizuto³  Celso K. Sonoda³  Karoline Reich⁴  Toni Dobsak⁴  Stefan Tangl⁴ 

[1] Department of Anatomy, Araçatuba Dental School, UNESP-Univ Estadual Paulista, São Paulo, Brazil;Department of Preventive, Restorative and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland;Department of Oral Biology, Medical University of Vienna, Vienna, Austria;Department of Surgery and Integrated Clinic, Araçatuba Dental School, UNESP-Univ Estadual Paulista, São Paulo, Brazil;Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Division of Oral Surgery, Medical University of Vienna, Vienna, Austria;Austrian Cluster for Tissue Regeneration, Vienna, Austria;

关键词: Bone substitutes;    Bone formation;    Bone regeneration;    Bone augmentation;    Platelet-derived growth factor-BB;    Rat calvaria defects;    β-tricalcium phosphate;    Demineralized bovine bone mineral;    Histomorphometry;    Graft consolidation;   

DOI  :  10.1186/s12903-016-0210-3

 received in 2015-10-02, accepted in 2016-04-21,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundSupplementation of bone substitutes with recombinant platelet-derived growth factor-BB (PDGF-BB) can enhance bone regeneration. The aim of the study was to evaluate the effect of PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix in a rat calvaria defect model.MethodsThe authors examined 5 mm rat calvarial defects treated with β-tricalcium phosphate (TCP) or demineralized bovine bone mineral (DBBM) with and without 0.3 mg/ml recombinant PDGF-BB. Calvaria defects were randomly divided into the following treatment groups (n = 5); TCP; TCP plus PDGF-BB; DBBM; DBBM plus PDGF-BB; and untreated empty control. After 45 days, bone formation was evaluated by histomorphometry and fluorescence microscopy.ResultsThe authors report that the area of newly formed bone was similar between the empty controls and the two bone substitutes, TCP and DBBM. Supplementation of TCP and DBBM with PDGF-BB had no significant impact on bone formation. Fluorochrome staining revealed no visible changes in the pattern of bone formation in defects filled with TCP and DBBM, irrespective of PDGF-BB. Furthermore, supplementation with PDGF-BB did not influence biomaterial degradation.ConclusionsThe authors concluded that PDGF-BB had no impact on bone formation and degradation of bone substitutes in the respective rodent models. Thus, possible beneficial effects of PDGF-BB may require other model situations.

【 授权许可】

CC BY   
© Luvizuto et al. 2016