BMC Complementary and Alternative Medicine | |
The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model | |
Research Article | |
Lu Wang1  Jiadao Yong1  Yaping Wang1  Yanyan Zhang1  Pengwei Jing1  Wenxu Hu2  | |
[1] Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, No.1 Yixueyuan Road, 400016, Yuzhong District, Chongqing, China;Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, No.1 Yixueyuan Road, 400016, Yuzhong District, Chongqing, China;Department of stomatology and oral & maxillofacial surgery, YongChuan Hospital, Chongqing Medical University, 402160, Chongqing, China; | |
关键词: Hematopoietic; Bone marrow; Stem cell; BMSC; D-galactose; Aging; Senescence; Ginsenoside Rg1; Ginseng; | |
DOI : 10.1186/s12906-015-0642-3 | |
received in 2014-11-03, accepted in 2015-04-07, 发布年份 2015 | |
来源: Springer | |
【 摘 要 】
BackgroundGinsenoside Rg1 (Rg1) is one of the most active ingredients in Panax ginseng and has been proven to have anti-oxidative and anti-aging properties. However, there have been few reports concerning the anti-aging effects of Rg1 on the hematopoietic microenvironment and bone marrow stromal cells (BMSCs).MethodsThirty Sprague-Dawley rats were randomly divided into four groups (control, D-galactose (D-gal)-administration, Rg1-treatment, and D-gal-administration + Rg1-treatment groups). After D-gal and Rg1 treatment, BMSCs were extracted from femoral bone marrow for culture. After three passages, BMSCs were tested by senescence-associated β-galactosidase (SA-β-gal) staining, flow cytometric cell cycle phase distribution assay, CCK-8 cell proliferation assay, oxidative stress (reactive oxygen species [ROS], superoxide dismutase [SOD], and malondialdehyde [MDA]) assays, inflammatory marker (interleukin (IL)-2, IL-6, and tumor necrosis factor (TNF)-α) enzyme-linked immunosorbent assay (ELISA), stem cell factor (SCF) ELISA, and senescence-associated protein (p16, p21, and p53) Western blotting.ResultsCompared to the D-gal-administration group, the D-gal-administration + Rg1-treatment group showed significantly decreased levels of SA-β-gal + cell %, ROS, MDA, inflammatory marker expression, and senescence-associated protein expression as well as significantly increased levels of S-phase %, cell proliferation, SOD activity, and SCF expression. Compared to controls, the Rg-1-treatment group displayed significantly reduced levels of SA-β-gal + cell %, G1 phase %, ROS, MDA, inflammatory marker expression, senescence-associated protein expression, and SCF expression as well as significantly increased levels of S-phase %, cell proliferation, and SOD activity.ConclusionsRg1 improves the anti-aging ability of hematopoietic microenvironment through enhancing the anti-oxidant and anti-inflammatory capacities of BMSCs.
【 授权许可】
Unknown
© Hu et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
【 预 览 】
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