Multimodal in vivoimaging reveals limited allograft survival, intrapulmonary cell trapping and minimal evidence for ischemia-directed BMSC homing

BMC Biotechnology

Research Article

Annemie Van Der Linden¹  Jean-Pierre Timmermans²  Bert R Everaert³  Christiaan J Vrints⁴  Peter Ponsaerts⁵  Irene Bergwerf⁵  Nathalie De Vocht⁶ 

[1] BioImaging Laboratory, University of Antwerp, Antwerp, Belgium;Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium;Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium;Laboratory of Cellular and Molecular Cardiology, Antwerp University Hospital, Antwerp, Belgium;Laboratory of Cell Biology and Histology, Groenenborgerlaan 171, 2020, Antwerp, Belgium;Laboratory of Cellular and Molecular Cardiology, Antwerp University Hospital, Antwerp, Belgium;Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium;Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium;BioImaging Laboratory, University of Antwerp, Antwerp, Belgium;

关键词: Stem cell;    BMSC;    Homing;    Bioluminescence;    Confocal endomicroscopy;   

DOI  :  10.1186/1472-6750-12-93

 received in 2012-06-25, accepted in 2012-11-11,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundDespite positive reports on the efficacy of stem cell therapy for the treatment of cardiovascular disease, the nature of stem cell homing to ischemic tissues remains elusive.ResultsWe used a mouse model of peripheral tissue ischemia to study the survival and homing capacity of dual reporter gene (eGFP/Luciferase) expressing bone marrow-derived stromal cells (BMSC). Cell homing and survival were studied in the presence and absence of ciclosporin A (CsA) immunosuppression using bioluminescence imaging (BLI) together with confocal endomicroscopy. Different injection strategies were applied: central venous (CV), intra-arterial (IA) and intramuscular (IM). BLI and confocal endomicroscopy evidenced complete rejection of the IM injected allogeneic BMSC transplant within 5 to 10 days. Immunosuppression with CsA could only marginally prolong graft survival. IM injected BMSC did not migrate to the site of the arterial ligation. CV injection of BMSC resulted in massive pulmonary infarction, leading to respiratory failure and death. Intrapulmonary cell trapping was evidenced by confocal endomicroscopy, BLI and fluorescence microscopy. IA injection of BMSC proved to be a feasible and safe strategy to bypass the lung circulation. During the follow-up period, neither BLI nor confocal endomicroscopy revealed any convincing ischemia-directed homing of BMSC.ConclusionsBLI and confocal endomicroscopy are complementary imaging techniques for studying the in vivo biology of dual reporter gene-expressing BMSC. Allogeneic BMSC survival is limited in an immunocompetent host and cannot be preserved by CsA immunosuppression alone. We did not find substantial evidence for ischemia-directed BMSC homing and caution against CV injection of BMSC, which can lead to massive pulmonary infarction.

【 授权许可】

CC BY   
© Everaert et al.; licensee BioMed Central Ltd. 2012