Evaluation of the in vitro antibacterial activity of the solvent fractions of the leaves of Rhamnus prinoides L’Herit (Rhamnaceae) against pathogenic bacteria

BMC Complementary and Alternative Medicine

Research Article

Haile Alemayehu¹  Getachew Tadesse²  Teshome Nedi³  Workineh Shibeshi³  Yalew Molla⁴ 

[1] Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P. O. Box, 1176, Addis Ababa, Ethiopia;Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, P. O. Box, 1176, Addis Ababa, Ethiopia;Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Addis Ababa University, P. O. Box, 1176, Addis Ababa, Ethiopia;Department of Pharmacy, College of Health Sciences, Debre Markos University, P. O. Box, 269, Debre Markos, Ethiopia;

关键词: Antibacterial activity;    Minimum inhibitory concentration;    Staphylococcus aureus;    Streptococcus pyogen;    Streptococcus pneumoniae;    Salmonella typhi;   

DOI  :  10.1186/s12906-016-1279-6

 received in 2016-03-23, accepted in 2016-08-11,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMedicinal plants play great roles in the treatment of various infectious diseases. Rhamnus prinoides is one of the medicinal plants used traditionally for treatment of bacterial diseases. The antibacterial activity of the crude extract of the plant had been shown by a previous study, but this study was undertaken to further the claimed medicinal use of the plant by screening its solvent fractions for the said activity so that it could serve as a basis for subsequent studies.MethodsThe solvent fractions of the plant were obtained by successive soxhlet extraction with solvents of increasing polarity, with chloroform and methanol, followed by maceration of the marc of methanol fraction with water. The antibacterial activity of the solvent fractions was evaluated on seven bacterial species using agar well diffusion method at different concentrations (78 mg/well, 39 mg/well and 19.5 mg/well) in the presence of positive and negative controls. The minimum inhibitory concentration of the solvent fractions was determined by micro-broth dilution method using resazurin as indicator.ResultMethanol and chloroform fractions revealed antibacterial activities against the growth of test bacterial strains with varying antibacterial spectrum and the susceptible bacterial species were Staphylococcus aureus, Streptococcus pyogen, Streptococcus pneumoniae and Salmonella typhi. The average minimum inhibitory concentration value of the methanol and chloroform fractions ranged from 8.13 mg/ml to 32.5 mg/ml and from 8.13 mg/ml to 16.25 mg/ml, respectively.ConclusionThe methanol and chloroform fractions demonstrated significant antibacterial activities against the growth of pathogenic bacteria but the aqueous fraction did not reveal antibacterial activity against any of the test bacteria.

【 授权许可】

CC BY   
© The Author(s). 2016