Bactericidal Activity of Silver Nanoparticles Produced by Fusarium solani against the Multidrug-Resistant Bacteria.

Research Journal of Pharmaceutical, Biological and Chemical Sciences

article

Basma H. Amin¹  Reyad Mohamed El-Sharkawy.² 

[1] The Regional Centre for Mycology and Biotechnology, Al-Azhar University;Botany and Microbiology Department, Faculty of Science, Benha University

关键词: Fusarium solani;    Silver nanoparticle;    Multidrug-resistant bacteria;    Antibacterial activity;    Minimum inhibitory concentration;    Bacterial cell ultrastructure.;   

DOI  :  10.33887/rjpbcs/2019.10.6.25

学科分类：工程和技术（综合）

来源: Research Journal of Pharmaceutical, Biological and Chemical Sciences

【 摘 要 】

The recent emergence of novel therapeutic agents replacing traditional antibiotics has receivedtremendous attention due to their unique efficiency against the quick developed multidrug-resistancepathogenic bacteria. In the present study, the biosynthesis of silver nanoparticles (AgNPs) by Fusarium solaniand their antibacterial efficiency against different multidrug-resistant bacteria was investigated. The reducingcapability of silver ions to AgNPs by F. solani was confirmed by UV–visible spectroscopy showing a surfaceplasmon resonance of 420 nm and Energy dispersive X-ray analysis. The biosynthetic nanosilver weremonodispersed, spherical to oval shape, irregular in shape with an average molecular size of 6–15 nm and highnegative charge as revealed from Transmission electron microscopy and Zeta analyses. The antibacterial effectof nanosilver was examined against the investigated multidrug resistant bacteria, Bacillus subtilis, methicillin- resistant Staphylococcus aureus, Escherichia coli and Klebsiella pneumonia. The developed AgNPs had asignificant antibacterial activity on all tested bacterial isolates. The bactericidal effect of AgNPs was highlyconcentration-dependent and the minimum inhibitory concentration of AgNPs against MDR bacteria was 40ppm. Notably, the antibacterial susceptibility of Gram-positive bacteria was higher when compared with thatof Gram-negative bacteria as confirmed by the reduction in bacterial growth and the morphological changesoccurred in bacterial cells ultrastructure. Thus, the fungus F. solani can be considered a potential bio-factoryfor the biosynthesis of AgNPs with a remarkable bactericidal activity against the examined multidrug resistancepathogenic bacteria.

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