Isolation and identification of Endophytic Fungus Fusarium sp from Agarwood (Aquilaria sp) population originated from the forest of Aceh Tamiang district, Indonesia

8th Annual International Conference 2018 on Science and Engineering

工业技术（总论）

Zulfendi^1^2 ; Idroes, Rinaldi^3^4 ; Khairan^3

Graduate School of Mathematics and Applied Sciences, Syiah Kuala University, Kopelma Darussalam, Banda Aceh

23111, Indonesia^1

Human Resources Development Agency, Banda Aceh, Aceh Province, Indonesia^2

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Kopelma Darussalam, Banda Aceh

23111, Indonesia^3

Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Kopelma Darussalam, Banda Aceh

23111, Indonesia^4

关键词: Biological response;    Endophytic fungi;    Fungal infection;    Fungal isolates;    Fusarium oxysporums;    Fusarium solani;    Isolation and identification;    Streptomyces sp;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/523/1/012013/pdf
DOI  :  10.1088/1757-899X/523/1/012013

学科分类：工业工程学

来源: IOP

【 摘 要 】

Aquilaria sp is one of higher plant types that can produce agarwood. Agarwood is commonly stimulated by the biological response of endophytic fungal infection of Fusarium sp. This research aims to isolate and identify endophytic fungus Fusarium sp from Aquilaria sp populations originated from the forest of Aceh Tamiang District. The pure isolates of Fusarium sp derived from Aquilaria sp that reinfected on Aquilaria sp populations to stimulate agarwood formation. The endophytic fungal isolates of Fusarium sp were growth on a selective media of Sabouraud Dextrose Agar (SDA) containing antibiotics Streptomycin 10 g/dl and Chloramphenicol 25 g/dl. Ten percent of infected Aquilaria sp from the population was taken aseptically. Isolation and identification of endophytic fungus Fusarium sp were conducted from June to August 2016. The results showed that within 10 infected Aquilaria sp, several microbe species were found such as three Fusarium sp, six Aspergillus sp, two Rhizopus sp, two Penicillium sp, one Bacillus sp, one Actinomyces sp and one Streptomyces sp species. The result of identification towards three species of endophytic fungus Fusarium sp that has been purified were srongly suspected to be Fusarium solani, Fusarium oxysporum, and Fusarium monyliformae.